Vorob'eva G S, Pechurkin N S
Antibiot Med Biotekhnol. 1985 Jul;30(7):521-5.
The physicochemical properties of a model medium consisting of two substances, sodium sulfite and albumin, were studied. It was shown that the presence of a SAS in the sulfite solution significantly lowered the rate of oxygen sorption by the model medium. The higher the SAS concentration, the lower the sorption rate. When yeasts were grown on sulfite liquor under conditions of the reduced medium the rate of their growth and respiration did not significantly depend on aeration within wide ranges of its variation. The experiments with the model medium demonstrated that the aeration independence was due to indifference to oxygen not of the cells but of the medium as the result of its physicochemical properties. The effect of sorption hysteresis was also studied on the model medium. It was revealed that the rate of oxygen desorption from the medium was lower than that of oxygen sorption by the same medium. The effect was not observed when a nonionic SAS was added to the medium and the isoelectric point was almost reached. The effect was connected with impairment of oxygen diffusion through the medium surface film. Interaction of the substances included in the model medium was of physical nature.
对由亚硫酸钠和白蛋白两种物质组成的模型介质的物理化学性质进行了研究。结果表明,亚硫酸盐溶液中表面活性剂的存在显著降低了模型介质对氧气的吸附速率。表面活性剂浓度越高,吸附速率越低。当酵母在还原培养基条件下于亚硫酸盐溶液中生长时,在较大的通气变化范围内,其生长和呼吸速率并不显著依赖于通气。对模型介质进行的实验表明,通气独立性并非由于细胞对氧气不敏感,而是由于介质的物理化学性质导致其对氧气不敏感。还研究了模型介质上的吸附滞后效应。结果发现,介质中氧气的解吸速率低于相同介质对氧气的吸附速率。当向介质中加入非离子表面活性剂且几乎达到等电点时,未观察到该效应。该效应与氧气通过介质表面膜的扩散受损有关。模型介质中所含物质之间的相互作用具有物理性质。