Vaĭnberg Iu P, Isaeva L V, Kir'ianov G I, Shagalov L B
Antibiot Med Biotekhnol. 1985 Jul;30(7):532-4.
The complex of the tritium labeled DNA with carminomycin was injected intravenously to mice. Five h after the complex injection the rats were sacrificed. The liver was isolated and homogenized. The cells were consecutively divided into fractions: intercellular liquid, cytoplasm, nuclear juice and chromatin. The content of high-molecular exogenous DNA and the products of its degradation, as well as the quantity of free and DNA bound carminomycin were determined radiometrically and spectrofluorometrically in every of the above fractions. It was found that the carminomycin complex located in the liver remained unchanged for a long time after injection and penetrated into the cell in the form of the complex. In the cytoplasm, the complex disintegrated to liberate carminomycin which penetrated into the nucleus where it was bound to chromatin DNA.
将氚标记的DNA与柔红霉素的复合物静脉注射到小鼠体内。注射复合物5小时后处死大鼠。分离肝脏并匀浆。细胞依次分为细胞间液、细胞质、核液和染色质部分。用放射性测定法和荧光分光光度法测定上述各部分中高分子量外源DNA及其降解产物的含量，以及游离和与DNA结合的柔红霉素的量。结果发现，注射后肝脏中存在的柔红霉素复合物长时间保持不变，并以复合物的形式进入细胞。在细胞质中，复合物分解以释放出柔红霉素，柔红霉素进入细胞核并与染色质DNA结合。
