粪便氨基酸稳定性：探究分析的最佳采样条件
Faecal amino acids stability: investigating optimal sampling conditions for analysis.
作者信息
Opperman Roza C M, Vermeer Eva, Bosch Sofie, de Meij Tim G J, de Boer Nanne K H, Struys Eduard A
机构信息
Department of Gastroenterology and Hepatology, Amsterdam University Medical Centre, Vrije Universiteit Amsterdam, Amsterdam, 1081 HV, The Netherlands.
Amsterdam Gastroenterology Endocrinology Metabolism (AGEM) Research Institute, Amsterdam University Medical Centre, Amsterdam, The Netherlands.
出版信息
Metabolomics. 2025 Jun 14;21(4):81. doi: 10.1007/s11306-025-02279-3.
INTRODUCTION
Faecal amino acids are promising non-invasive diagnostic biomarkers, but stability remains unclear.
OBJECTIVES
This study examined whether storage conditions, sampling site within the sample, freeze-thaw cycles, and the OMNImet·GUT device affect faecal amino acid concentrations.
METHODS
Faecal samples from three donors underwent various pre-analytical conditions. Amino acids were analysed through targeted liquid chromatography-tandem mass spectrometry.
RESULTS
Most amino acids remained stable across sampling sites and freeze-thaw cycles. Storage at -20 °C preserved integrity, whereas 4 °C and 20 °C storage led to variations. The OMNImet·GUT device stabilised some amino acids but showed inconsistencies.
CONCLUSION
Pre-analytical conditions influence faecal amino acid concentrations. Standardisation is essential for biomarker reliability.
引言
粪便氨基酸是很有前景的非侵入性诊断生物标志物，但稳定性仍不明确。
目的
本研究考察了储存条件、样本内采样部位、冻融循环以及OMNImet·GUT设备是否会影响粪便氨基酸浓度。
方法
来自三名捐赠者的粪便样本经历了各种分析前条件。通过靶向液相色谱-串联质谱法分析氨基酸。
结果
大多数氨基酸在不同采样部位和冻融循环中保持稳定。在-20°C储存可保持完整性，而在4°C和20°C储存会导致变化。OMNImet·GUT设备使一些氨基酸稳定，但存在不一致性。
结论
分析前条件会影响粪便氨基酸浓度。标准化对于生物标志物的可靠性至关重要。
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