You Hongjuan, Zheng Chunfu
Department of Pathogenic Biology and Immunology, Jiangsu Key Laboratory of Immunity and Metabolism, Xuzhou Medical University, Xuzhou, Jiangsu, China.
Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.
Methods Mol Biol. 2025;2940:27-32. doi: 10.1007/978-1-0716-4615-1_4.
Virus-host interactions play crucial roles throughout the entire process of viral replication. Specific viral proteins often target key proteins of the host. These interactions can be identified through various experimental techniques. Co-immunoprecipitation (co-IP) is a powerful method for detecting and characterizing protein-protein interactions and understanding their functional significance. In this context, Co-IP is commonly used to screen and identify viral proteins that interact with host proteins, providing valuable insights into the mechanisms of viral replication.
病毒与宿主的相互作用在病毒复制的整个过程中起着至关重要的作用。特定的病毒蛋白通常靶向宿主的关键蛋白。这些相互作用可以通过各种实验技术来识别。免疫共沉淀（co-IP）是一种检测和表征蛋白质-蛋白质相互作用并了解其功能意义的强大方法。在这种情况下，免疫共沉淀通常用于筛选和鉴定与宿主蛋白相互作用的病毒蛋白，为病毒复制机制提供有价值的见解。
