Suppr超能文献

通过免疫共沉淀(Co-IP)分析病毒与宿主的蛋白质-蛋白质相互作用。

Analysis of the Virus-Host Protein-Protein Interaction via Co-Immunoprecipitation (Co-IP).

作者信息

You Hongjuan, Zheng Chunfu

机构信息

Department of Pathogenic Biology and Immunology, Jiangsu Key Laboratory of Immunity and Metabolism, Xuzhou Medical University, Xuzhou, Jiangsu, China.

Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.

出版信息

Methods Mol Biol. 2025;2940:27-32. doi: 10.1007/978-1-0716-4615-1_4.

Abstract

Virus-host interactions play crucial roles throughout the entire process of viral replication. Specific viral proteins often target key proteins of the host. These interactions can be identified through various experimental techniques. Co-immunoprecipitation (co-IP) is a powerful method for detecting and characterizing protein-protein interactions and understanding their functional significance. In this context, Co-IP is commonly used to screen and identify viral proteins that interact with host proteins, providing valuable insights into the mechanisms of viral replication.

摘要

病毒与宿主的相互作用在病毒复制的整个过程中起着至关重要的作用。特定的病毒蛋白通常靶向宿主的关键蛋白。这些相互作用可以通过各种实验技术来识别。免疫共沉淀(co-IP)是一种检测和表征蛋白质-蛋白质相互作用并了解其功能意义的强大方法。在这种情况下,免疫共沉淀通常用于筛选和鉴定与宿主蛋白相互作用的病毒蛋白,为病毒复制机制提供有价值的见解。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验