Dai Jiaqi, Gong Zuxin, Li Xin, Liu Chenchen, Zheng Chunfu, Liu Gongguan
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu, China.
Department of Microbiology, Immunology & Infectious Diseases, University of Calgary, Calgary, AB, Canada.
Methods Mol Biol. 2025;2940:133-140. doi: 10.1007/978-1-0716-4615-1_12.
Pull-down techniques are essential for identifying direct protein-protein interactions in vitro and play a pivotal role in the study of virus-host protein interactions. These methods rely on a bait protein immobilized on a solid support to capture target prey proteins, which are subsequently eluted and identified. In the context of viral infection research, pull-down techniques offer high specificity and low background interference, which are crucial for elucidating protein interaction networks and investigating cellular signaling pathways. This chapter provides a detailed description of the experimental methods for validating virus-host protein interactions through pull-down techniques, offers vital insights into the molecular mechanisms underlying viral infections, and serves as a cornerstone for drug development and therapeutic strategies.
下拉技术对于在体外鉴定直接的蛋白质-蛋白质相互作用至关重要，并且在病毒-宿主蛋白质相互作用的研究中发挥着关键作用。这些方法依赖于固定在固体支持物上的诱饵蛋白来捕获目标猎物蛋白，随后将其洗脱并鉴定。在病毒感染研究的背景下，下拉技术具有高特异性和低背景干扰，这对于阐明蛋白质相互作用网络和研究细胞信号通路至关重要。本章详细描述了通过下拉技术验证病毒-宿主蛋白质相互作用的实验方法，深入洞察了病毒感染背后的分子机制，并为药物开发和治疗策略奠定了基础。
