Liang Weizheng, Wang Peng, Wang Xiran, Xu Dandan, Zheng Chunfu
Hebei Key Laboratory of Systems Biology and Gene Regulation, Central Laboratory, The First Affiliated Hospital of Hebei North University, Zhangjiakou, Hebei, China.
Hebei North University, Zhangjiakou, Hebei, China.
Methods Mol Biol. 2025;2940:93-107. doi: 10.1007/978-1-0716-4615-1_9.
Single-cell RNA sequencing (scRNA-seq) is a revolutionary technology capable of analyzing host and viral transcripts and host-virus interactions at the single-cell level. Viral infection is a complex process involving interactions between the virus and the host cell. While traditional research methods often have difficulty revealing cellular heterogeneity and dynamics during viral infections, scRNA-seq technology, which enables the sequencing of individual cells, provides a powerful tool for the in-depth study of viral-host interactions. scRNA-seq was able to reveal the mechanism of molecular changes in host cells during viral infection, providing new ideas and methods for the diagnosis and treatment of viral infections. This article describes the application, advantages, and experimental procedures of scRNA-seq in host virology.
单细胞RNA测序（scRNA-seq）是一项革命性技术，能够在单细胞水平分析宿主和病毒转录本以及宿主-病毒相互作用。病毒感染是一个复杂的过程，涉及病毒与宿主细胞之间的相互作用。虽然传统研究方法在揭示病毒感染期间的细胞异质性和动态变化方面往往存在困难，但能够对单个细胞进行测序的scRNA-seq技术为深入研究病毒-宿主相互作用提供了强大工具。scRNA-seq能够揭示病毒感染期间宿主细胞分子变化的机制，为病毒感染的诊断和治疗提供了新的思路和方法。本文介绍了scRNA-seq在宿主病毒学中的应用、优势及实验流程。
