Keppel Patrick, Hecko Sebastian, Mikula Hannes
Institute of Applied Synthetic Chemistry, TU Wien, Getreidemarkt 9, 1060, Vienna, Austria.
Top Curr Chem (Cham). 2025 Jun 14;383(3):25. doi: 10.1007/s41061-025-00492-1.
Bioorthogonal bond-cleavage reactions have evolved into powerful tools for chemical biology, representing a promising strategy for achieving controlled release of molecules under physiologically relevant conditions, even in living organisms. Since their discovery, significant efforts have been invested in the development and understanding of the underlying chemistries to enhance the click-to-release performance, biocompatibility, and stability of bioorthogonal tools. In this review, we aim to provide a concise overview of click-triggered bioorthogonal bond-cleavage reactions, with an emphasis on the mechanisms and characteristics of the most commonly applied click-to-release chemistries.
生物正交键断裂反应已发展成为化学生物学的强大工具，是在生理相关条件下，甚至在活生物体中实现分子可控释放的一种有前景的策略。自其被发现以来，人们在开发和理解其基础化学方面投入了大量精力，以提高生物正交工具的点击释放性能、生物相容性和稳定性。在本综述中，我们旨在简要概述点击触发的生物正交键断裂反应，重点介绍最常用的点击释放化学的机制和特点。
