Ouyang Huilin, Zentek Jürgen, Ebersbach Luisa, Celeiro María, Lores Marta, Martínez-Vallespín Beatriz
Institute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität Berlin, Königin-Luise-Str. 49, Berlin 14195, Germany.
Institute of Animal Nutrition, Department of Veterinary Medicine, Freie Universität Berlin, Königin-Luise-Str. 49, Berlin 14195, Germany.
Poult Sci. 2025 May 30;104(9):105366. doi: 10.1016/j.psj.2025.105366.
The current study evaluated the effect of a white grape marc extract (GME) on growth performance, gut health, and intestinal function in broiler chickens at different dosages to determine a dose-related efficacy of the extract without inducing any adverse effects. Four hundred male broiler chickens received a basal diet without (CON) or with the GME at different levels, based on its polyphenolic concentration: 200 (LPP), 750 (MPP), and 1,500 (HPP) mg/kg, respectively. Growth performance parameters were recorded during the whole trial. At 35 days, apparent ileal digestibility (AID) of several nutrients was determined. Additionally, jejunal and cecal tissues were collected to assess tissue morphology. Jejunal tissue was also gathered for gene expression and Ussing chamber studies. Finally, breast muscle was collected for lipid oxidation assessment. Dietary supplementation of the GME did not significantly impact the growth performance of broiler chickens with the exception of the FCR that seemed to be increased by the GME supplementation (P = 0.083). The AID of most of the analyzed nutrients was also unaffected with the exception of the amino acid aspartic acid, which was higher in MPP and HPP compared to LPP (P = 0.006). No significant differences (P > 0.05) were found in villus length (VL), crypt depth (CD), villus-to-crypt ratio (V/C) in the jejunum, or in cecal CD. The number of goblet cells in jejunal villi and crypts was higher in MPP and HPP than in CON (P < 0.001). There were no effects on the mRNA abundance of some pro-inflammatory cytokines, mucins, or tight junction proteins measured in jejunal tissue nor on antioxidant capacity measured in breast muscle. In the Ussing chamber experiment, the GME decreased jejunal tissue conductance after glucose addition in MPP and HPP groups (P = 0.033). In conclusion, although the inclusion of a white GME in the diet of broiler chickens did not significantly enhance growth performance or nutrient absorption, it was confirmed that none of the tested concentrations induced adverse effects.
本研究评估了白葡萄渣提取物（GME）在不同剂量下对肉鸡生长性能、肠道健康和肠道功能的影响，以确定该提取物在不产生任何不良影响的情况下与剂量相关的功效。400只雄性肉鸡分别接受不含GME的基础日粮（CON）或根据其多酚浓度添加不同水平GME的日粮：分别为200（低多酚组，LPP）、750（中多酚组，MPP）和1500（高多酚组，HPP）mg/kg。在整个试验期间记录生长性能参数。在第35天，测定了几种营养素的回肠表观消化率（AID）。此外，收集空肠和盲肠组织以评估组织形态。还收集空肠组织用于基因表达和尤斯灌流小室研究。最后，收集胸肌进行脂质氧化评估。日粮添加GME对肉鸡生长性能没有显著影响，但饲料转化率（FCR）似乎因添加GME而有所增加（P = 0.083）。除天冬氨酸外，大多数分析营养素的AID也未受影响，天冬氨酸在MPP组和HPP组中的含量高于LPP组（P = 0.006）。空肠绒毛长度（VL）、隐窝深度（CD）、绒毛与隐窝比值（V/C）或盲肠CD均未发现显著差异（P > 0.05）。MPP组和HPP组空肠绒毛和隐窝中的杯状细胞数量高于CON组（P < 0.001）。对空肠组织中一些促炎细胞因子、粘蛋白或紧密连接蛋白的mRNA丰度以及胸肌中抗氧化能力均无影响。在尤斯灌流小室实验中，MPP组和HPP组添加葡萄糖后GME降低了空肠组织电导（P = 0.033）。总之，虽然在肉鸡日粮中添加白葡萄渣提取物没有显著提高生长性能或营养吸收，但证实所有测试浓度均未诱导不良影响。
