使用超滤器分析人体血液中的游离色氨酸：与其他方法的比较。

The analysis of free tryptophan in human blood with the Ultrafiltrator: a comparison with other methods.

作者信息

Baumann P, Perey M

出版信息

Clin Chim Acta. 1977 Apr 15;76(2):223-31. doi: 10.1016/0009-8981(77)90100-0.

Abstract

Recently, the Ultrafiltrator has been described, in the literature, which allows the ultrafiltration of small molecules in minute blood samples under anaerobic conditions with filters of any pore size. This apparatus has been tested for the separation of free tryptophan in heparinised and EDTA-plasma and in whole blood. At the same time, free tryptophan has been analysed after ultrafiltration with CF-50 A and CF-25 membrane cones (Centriflo). The results obtained by these two techniques are compared. Finally, a comprehensive review of other methods reported in the literature, concerning the isolation of free tryptophan, is presented.

摘要

最近，文献中描述了一种超滤器，它能够在厌氧条件下，使用任何孔径的过滤器对微量血样中的小分子进行超滤。该仪器已针对在肝素化血浆、乙二胺四乙酸（EDTA）血浆和全血中分离游离色氨酸进行了测试。同时，还使用CF - 50 A和CF - 25膜锥（Centriflo）进行超滤后对游离色氨酸进行了分析。对这两种技术所获得的结果进行了比较。最后，对文献中报道的关于游离色氨酸分离的其他方法进行了全面综述。