[血浆中游离色氨酸和总色氨酸的荧光测定法]
[Fluorometric determination of free and total tryptophan in blood plasma].
作者信息
Escande C, Bousquet B, Dreux C
出版信息
Ann Biol Clin (Paris). 1977;35(5):387-95.
Free and total tryptophan is estimated in the plasma by a spectrofluorimetric method using an amplification reaction and fluorescence by transformation of tryptophan into norharman using formalin and perhydrol (fluorescence is amplified about 15 times). Free tryptophan in the plasma is separated by ultrafiltration under pressure. We verified the analytical characteristics of the method in order to obtain optimal conditions. Values frequently found in 16 blood donors in good health were 10.4 +/- 3.3 micronmol/l. for free tryptophan (average +/- ts for a risk of 5%) and 54.6 +/- 18.6 micronmol/l. for total tryptophan.
采用荧光分光光度法，通过放大反应，利用福尔马林和双氧水将色氨酸转化为去甲哈尔满产生荧光（荧光放大约15倍）来测定血浆中的游离色氨酸和总色氨酸。血浆中的游离色氨酸通过加压超滤进行分离。我们验证了该方法的分析特性以获得最佳条件。16名健康献血者的游离色氨酸值通常为10.4±3.3微摩尔/升（平均±5%风险的标准误），总色氨酸值为54.6±18.6微摩尔/升。
Zotero 插件