Lao Shuqun, Wang Haiyang, Wu Di, Wei Qiaohua, Tang Dianping
Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou, 350108, China.
Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou, 350108, China.
Biosens Bioelectron. 2025 Nov 1;287:117689. doi: 10.1016/j.bios.2025.117689. Epub 2025 Jun 11.
Due to unique framework structures, Prussian blue analogs (PBAs) have long been of considerable interest in biosensing. However, the predominantly cubic morphology of most PBAs, along with their inherent elemental and structural limitations, restricts their catalytic performance as the nanozymes. Herein, we designed a dual-metal CuFe/Fe PBA nanozyme composite (CuFe/Fe DMPBA) exhibiting excellent peroxidase (POD)-like activity. The synthesized FePBA was prismatically loaded along the inner core CuFe PBA to build an epitaxially deposited structure. This unique architecture resulted in a substantial enhancement of the POD-like activity of the composite nanozyme compared to its individual components, FePBA and CuFe PBA. Leveraging the superior catalytic performance of the synthesized CuFe/Fe DMPBA, we further developed a highly sensitive and selective colorimetric immunoassay platform for the detection of human epidermal growth factor receptor 2 (HER2), a crucial biomarker of cancer. Under optimized conditions, this nanozyme-based immunoassay platform achieved a limit of detection (LOD) of 0.007 ng mL and a linear range from 0.01 to 10 ng mL for HER2 detection. This work not only presents a novel strategy for synthesizing high-performance PBA-based nanozymes, but also provides insights for the rational design and application of PBA materials in bioanalytical sensing.
由于独特的框架结构，普鲁士蓝类似物（PBAs）长期以来在生物传感领域备受关注。然而，大多数PBAs主要为立方形态，以及其固有的元素和结构局限性，限制了它们作为纳米酶的催化性能。在此，我们设计了一种具有优异过氧化物酶（POD）样活性的双金属CuFe/Fe PBA纳米酶复合材料（CuFe/Fe DMPBA）。合成的FePBA沿内核CuFe PBA呈棱柱形负载，以构建外延沉积结构。与单独的组分FePBA和CuFe PBA相比，这种独特的结构导致复合纳米酶的POD样活性大幅增强。利用合成的CuFe/Fe DMPBA的优异催化性能，我们进一步开发了一种用于检测人表皮生长因子受体2（HER2）的高灵敏度和选择性比色免疫分析平台，HER2是一种关键的癌症生物标志物。在优化条件下，这种基于纳米酶的免疫分析平台对HER2检测的检测限（LOD）为0.007 ng/mL，线性范围为0.01至10 ng/mL。这项工作不仅提出了一种合成高性能基于PBA的纳米酶的新策略，还为PBA材料在生物分析传感中的合理设计和应用提供了见解。
