Hong Jun Seon, Yu Myunghwan, Nawarathne Shan Randima, Oketch Elijah Ogola, Yun Hyeonho, Jayasena Dinesh Darshaka, Heo Jung Min
Department of Animal Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
Technical Marketing Division, Protein Solution Business Unit, CJ CheilJedang Bio, Seoul 04560, Korea.
J Anim Sci Technol. 2025 May;67(3):592-606. doi: 10.5187/jast.2024.e39. Epub 2025 May 31.
The experiment was conducted to estimate the matrix value of xylanase and the effect of dietary xylanase supplementation on growth performance, viscosity, digestibility, and carcass traits in broiler chickens. A total of 588 one-day-old Ross 308 broiler chicks were raised with a commercial diet until day 7 and seven-day-old chicks were randomly allotted to one of seven dietary treatments with twelve replicates. Diets were corn-soybean meat based with wheat added. Dietary treatments were as follows; four basal diets (PC, energy sufficient diet; NC-1, -40 kcal/kg ME reduced from PC diet; NC-2, -80 kcal/kg ME reduced from PC diet; NC-3, -120 kcal/kg ME reduced from PC diet) and three different xylanase activity levels diet (NCX-1, 1,500 U/kg xylanase activity; NCX-2, 3,000 U/kg xylanase activity, NCX-3 4,500 U/kg xylanase activity) in the NC-3 diet. The standard xylanase dose was decided from the previous experiment. The weight gain and feed intake were measured and feed efficiency was calculated weekly. One bird per pen was selected and euthanized to harvest the intestinal digesta, breast meat, and leg meat samples on days 24 and 35. The linear and quadratic regression analysis and regression plateau were used to determine the xylanase recommendation and marginal level. The viscosity, digestibility, and proximate analysis of meat were analyzed from taken samples. Xylanase-added treatments were performed for higher ( < 0.05) body weight and body weight gain. Furthermore, xylanase-added treatments showed higher protein digestibility and lower viscosity compared to non-xylanase treatment. The maximum metabolizable energy compensation level of xylanase calculated by the regression was 120 kcal/kg and the marginal xylanase level showed maximum performances were 3,622 U/kg on the linear plateau and 4,000 U/kg on the quadratic plateau. Therefore, our experiment suggested that xylanase addition in an energy deficiency diet not only enhances growth performance but also reduces viscosity, and enhances protein digestibility and the maximum compensation level of metabolizable energy was 120 kcal/kg. The recommended levels of xylanase supplementation were determined to be 3,622 and 4,000 U/kg.
本试验旨在评估木聚糖酶的基质值,以及日粮添加木聚糖酶对肉鸡生长性能、粘度、消化率和胴体性状的影响。总共588只1日龄罗斯308肉鸡雏鸡用商品日粮饲养至7日龄,7日龄雏鸡随机分配到7种日粮处理中的一种,每种处理12个重复。日粮以玉米-豆粕型为主,并添加小麦。日粮处理如下:四种基础日粮(PC,能量充足日粮;NC-1,代谢能比PC日粮降低40 kcal/kg;NC-2,代谢能比PC日粮降低80 kcal/kg;NC-3,代谢能比PC日粮降低120 kcal/kg)和三种不同木聚糖酶活性水平的日粮(NCX-1,木聚糖酶活性1500 U/kg;NCX-2,木聚糖酶活性3000 U/kg;NCX-3,木聚糖酶活性4500 U/kg,添加于NC-3日粮中)。标准木聚糖酶剂量根据之前的试验确定。每周测量体重增加和采食量,并计算饲料效率。在第24天和第35天,每个重复栏选1只鸡进行安乐死,采集肠道食糜、胸肉和腿肉样本。采用线性和二次回归分析以及回归平台来确定木聚糖酶的推荐用量和边际水平。对采集的样本进行粘度、消化率和肉的常规分析。添加木聚糖酶的处理组体重和体重增加更高(P<0.05)。此外,与未添加木聚糖酶的处理相比,添加木聚糖酶的处理组蛋白质消化率更高,粘度更低。通过回归计算得出木聚糖酶的最大代谢能补偿水平为120 kcal/kg,在回归平台上,木聚糖酶的边际水平在生长性能达到最大值时,线性平台为3622 U/kg,二次平台为4000 U/kg。因此,我们的试验表明,在能量缺乏的日粮中添加木聚糖酶不仅能提高生长性能,还能降低粘度,提高蛋白质消化率,最大代谢能补偿水平为120 kcal/kg。木聚糖酶的推荐添加水平确定为3622和4000 U/kg。