Park Junhyun, Yogev Shaul
Neuroscience, Yale University, New Haven, Connecticut, United States.
MicroPubl Biol. 2025 May 29;2025. doi: 10.17912/micropub.biology.001547. eCollection 2025.
In studying the endomembrane system, organelle-specific markers tagged with fluorescent proteins are used to visualize individual organelles. However, whether the expression of organelle marker perturbs the organelle's biology is not always apparent. We report that expression of a GFP-tagged Endoplasmic Reticulum (ER) protein causes low levels of ER stress that are challenging to detect in control animals. This stress is revealed only once the ER-associated degradation (ERAD) pathway is compromised. Our results highlight the vulnerability of the ER and suggest that the possible contribution of ER stress to phenotypes obtained with transgenic markers should be considered when interpreting the phenotypes.
在研究内膜系统时，带有荧光蛋白标签的细胞器特异性标记物被用于可视化单个细胞器。然而，细胞器标记物的表达是否会干扰细胞器的生物学功能并不总是显而易见的。我们报告称，一种绿色荧光蛋白（GFP）标记的内质网（ER）蛋白的表达会导致低水平的内质网应激，而在对照动物中很难检测到这种应激。只有当内质网相关降解（ERAD）途径受损时，这种应激才会显现出来。我们的结果突出了内质网的脆弱性，并表明在解释表型时，应考虑内质网应激对通过转基因标记物获得的表型可能产生的影响。
