Eid Rony, Blochouse Estelle, Giese Alban, Papot Sébastien, Jay Philippe, Varon Christine, Poinot Pauline
University of Poitiers, UMR CNRS 7285, Institut de Chimie des Milieux et Matériaux de Poitiers (IC2MP), Cedex 9, Poitiers, 86073, France.
University of Bordeaux, INSERM, UMR1312 BoRdeaux Institute of Oncology, F33000, Bordeaux, France.
Biochem Biophys Rep. 2025 May 30;43:102062. doi: 10.1016/j.bbrep.2025.102062. eCollection 2025 Sep.
Induced volatolomics is an emerging field of research that offers new opportunities in biology by detecting volatile reporters released by activatable probes, enabling the exploration of oncogenic processes. Building on its proven efficiency in exploring the evolution of implanted tumours, we hypothesized that induced volatolomics could extend its application to detect precancerous conditions. As a proof of concept, we performed a longitudinal study and investigated glycosidase activity during the early stages of gastric carcinogenesis development induced by infection in mice, mimicking the gastric carcinogenesis cascade induced by chronic infection in humans. We identified upregulated exoglycosidases linked to acute infections or inflammatory processes in tissues infected by Helicobacter. Specifically, α-mannosidase and β-galactosidase activities in stomach tissue were found to be strongly associated with the initial stages of infection. Additionally, the activities of β-Glucuronidase and β-N-acetyl-glucosaminidase increased during the progression to preneoplastic stages, potentially signalling the transition from infection to inflammation-driven carcinogenesis. These enzymes may serve as early biomarkers for detecting gastric carcinogenesis. Our study highlights the potential of VOC-based probes for real-time monitoring of gastric cancer progression through tissue biopsies. Therefore, this study demonstrates the potential of induced volatolomics for investigating biological processes and uncovering new therapeutic strategies.
诱导挥发物组学是一个新兴的研究领域，通过检测可激活探针释放的挥发性报告物，为生物学研究提供了新的机会，有助于探索致癌过程。基于其在研究植入肿瘤演变方面已被证实的效率，我们推测诱导挥发物组学可以扩展其应用范围，用于检测癌前病变。作为概念验证，我们进行了一项纵向研究，调查了小鼠感染诱导的胃癌发生发展早期阶段的糖苷酶活性，模拟了人类慢性感染诱导的胃癌发生级联反应。我们在感染幽门螺杆菌的组织中鉴定出与急性感染或炎症过程相关的外切糖苷酶上调。具体而言，发现胃组织中的α-甘露糖苷酶和β-半乳糖苷酶活性与感染的初始阶段密切相关。此外，在进展到癌前阶段的过程中，β-葡萄糖醛酸酶和β-N-乙酰氨基葡萄糖苷酶的活性增加，这可能标志着从感染到炎症驱动的致癌作用的转变。这些酶可能作为检测胃癌发生的早期生物标志物。我们的研究强调了基于挥发性有机化合物的探针通过组织活检实时监测胃癌进展的潜力。因此·这项研究证明了诱导挥发物组学在研究生物学过程和揭示新治疗策略方面的潜力。
