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Simplification of 1H NMR spectra of proteins by one-dimensional multiple quantum filtration.

作者信息

Rance M, Dalvit C, Wright P E

出版信息

Biochem Biophys Res Commun. 1985 Sep 30;131(3):1094-102. doi: 10.1016/0006-291x(85)90203-7.

DOI:10.1016/0006-291x(85)90203-7
PMID:4052079
Abstract

The use of multiple quantum filters for simplification and editing of one-dimensional 1H NMR spectra of proteins is demonstrated. Three, four and five quantum-filtered spectra have been recorded. The technique is applicable to proteins of molecular weight up to at least 18,000. Examples obtained for a mixture of amino acids and for the proteins plastocyanin and myoglobin are reported. A remarkable degree of spectral editing can be achieved by judicious choice of experimental parameters.

摘要

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