Kawashima T, Halldin M M, Fukuyama K, Castagnoli N, Gellin G A, Epstein W L
Biochem Pharmacol. 1985 Oct 1;34(19):3517-21. doi: 10.1016/0006-2952(85)90726-9.
Intermediates of pheomelanin in tissue cultured B16 melanoma cells were analyzed by high performance liquid chromatography, and reduced glutathione (GSH), L-dopa, 2-[(L)-S-cysteinyl]-L-dopa (2-SCD) and 5-[(L)-S-cysteinyl]-L-dopa (5-SCD) were quantified. The effects of 4-tertiary butylcatechol (TBC), an antioxidant which causes skin depigmentation, on the levels of the intermediate were then examined. A concentration of 10(-4) M TBC increased the intracellular levels of GSH, 2-SCD and 5-SCD, whereas the L-dopa level was unchanged. The time-course of the increased intermediates corresponded to the elevation of glutathione-metabolizing enzyme activities previously reported by Kawashima et al. [J. invest. Derm. 82, 53 (1984)] in the same cell line exposed to 10(-4) M TBC. The findings establish chemical evidence that TBC stimulates pheomelanogenesis in melanocytes.
采用高效液相色谱法分析了组织培养的B16黑色素瘤细胞中褐黑素的中间体，并对还原型谷胱甘肽（GSH）、L-多巴、2-[(L)-S-半胱氨酰基]-L-多巴（2-SCD）和5-[(L)-S-半胱氨酰基]-L-多巴（5-SCD）进行了定量。然后研究了导致皮肤色素脱失的抗氧化剂4-叔丁基儿茶酚（TBC）对这些中间体水平的影响。10(-4) M的TBC浓度可提高细胞内GSH、2-SCD和5-SCD的水平，而L-多巴水平未发生变化。中间体增加的时间进程与Kawashima等人[《皮肤病学研究杂志》82, 53 (1984)]之前报道的在暴露于10(-4) M TBC的同一细胞系中谷胱甘肽代谢酶活性的升高相一致。这些发现提供了化学证据，表明TBC可刺激黑素细胞中的褐黑素生成。
