Yonemoto K, Gellin G A, Epstein W L, Fukuyama K
Biochem Pharmacol. 1983 Apr 15;32(8):1379-82. doi: 10.1016/0006-2952(83)90450-1.
Topical application of 4-tertiary butyl catechol (TBC) causes vitiligo in the skin of man and animals, and previous electron microscopic studies showed pheomelanin formation in the affected areas. In the present study, we investigated changes of enzyme activities, eumelanin content and amount of sulfur in tissue cultured human melanoma cells exposed to the depigmenting chemical. TBC enhanced glutathione reductase activity without changing the eumelanin content by 24 hr after exposure and subsequently (by 42 hr) increased gamma-glutamyl transpeptidase activity and sulfur content in the cells with a decrease in eumelanin content. It is suggested that this chemical alters the types of melanin formed by modulation of these enzyme activities.
局部应用4-叔丁基儿茶酚（TBC）会导致人和动物皮肤出现白癜风，先前的电子显微镜研究显示，在受影响区域会形成褐黑素。在本研究中，我们调查了暴露于这种色素脱失化学物质的人黑色素瘤组织培养细胞中酶活性、真黑素含量和硫含量的变化。TBC在暴露24小时后增强了谷胱甘肽还原酶活性，而真黑素含量没有改变，随后（42小时后）细胞中的γ-谷氨酰转肽酶活性和硫含量增加，同时真黑素含量降低。提示这种化学物质通过调节这些酶的活性来改变所形成的黑色素类型。
