Pilz I, Schwarz E, Palm W
Eur J Biochem. 1977 May 2;75(1):195-9. doi: 10.1111/j.1432-1033.1977.tb11517.x.
The conformation of the human immunoglobulin molecul Kol [IgG I, kappa2 gamma2, Gm(f)+] was studied by small-angle X-ray scattering in 0.15 M NaCl solution. The radius of gyration was found to be 5.84 +/- 0.04 nm, the volume 329 +/- 15 nm3 and the molecular weight 150 000 +/- 10 000. Information on the overall shape was obtained by comparing the experimental scattering curve with the calculated curves for various models. The models were obtained by arranging the models found for the Fab and Fc fragments of the same immunoglobulin molecule in a different manner. A model which fits all the date and the form of the experimental scattering curve is presented.
通过在0.15M NaCl溶液中进行小角X射线散射研究了人免疫球蛋白分子Kol[IgG I，kappa2 gamma2，Gm(f)+]的构象。发现回转半径为5.84±0.04nm，体积为329±15nm3，分子量为150000±10000。通过将实验散射曲线与各种模型的计算曲线进行比较，获得了关于整体形状的信息。这些模型是通过以不同方式排列同一免疫球蛋白分子的Fab和Fc片段的模型而获得的。提出了一个符合所有数据和实验散射曲线形式的模型。
