Orgambide G, Blangero C, Teissié J
Biochim Biophys Acta. 1985 Oct 24;820(1):58-62. doi: 10.1016/0005-2736(85)90215-9.
Electrofusion of Chinese hamster ovary cells is obtained with cells growing in monolayers on a culture dish. The cells were incubated with increasing amounts of ethanol before the field pulsation. The electroperforation was apparently not affected by the incubation but the threshold field intensity required to induce the fusion was shifted to higher values. This effect is tentatively explained by a decrease in size of the field-induced pores as a consequence of an increase in the lipid matrix fluidity.
中国仓鼠卵巢细胞的电融合是在培养皿中单层生长的细胞上实现的。在施加电场脉冲之前，细胞用逐渐增加量的乙醇进行孵育。电穿孔显然不受孵育的影响，但诱导融合所需的阈值场强向更高值偏移。这种效应初步解释为脂质基质流动性增加导致场致孔尺寸减小。
