Rols M P, Teissié J
Centre de Recherche de Biochimie et de Génétique Cellulaires du CNRS, Toulouse, France.
Biochemistry. 1990 May 15;29(19):4561-7. doi: 10.1021/bi00471a009.
Cells can be transiently permeabilized by application of high electric pulses of short duration. A direct consequence of this treatment is to induce a fusogenic state in the pulsed membrane. The molecular events underlying these phenomena remain to be explained. During our work, we investigated the effects of pulsing buffer osmotic pressure on both electric field induced permeabilization and fusion of Chinese hamster ovary cells growing either in monolayers or in suspension. Osmotic pressure has no effect on the induction step of permeabilization, but its increase was shown to inhibit the expansion step and to decrease the efficiency of the resealing phase. Fusion efficiency was greatly affected by the osmotic pressure and by the physiological state of the cells. When cells were grown plated and when intercellular contacts were spontaneous and present during pulsation, increasing the osmotic pressure resulted in an increase in the fusion index. The opposite effect was observed for cells growing in suspension and brought into contact after pulsation. These results were tentatively explained in terms of the effect of the osmotic pressure on the membrane organization and cell-cell contact quality.
通过施加短持续时间的高电脉冲，细胞可被瞬时通透化。这种处理的直接后果是在脉冲处理的细胞膜中诱导出融合状态。这些现象背后的分子事件仍有待解释。在我们的研究工作中，我们研究了脉冲缓冲液渗透压对单层生长或悬浮生长的中国仓鼠卵巢细胞的电场诱导通透化和融合的影响。渗透压对通透化的诱导步骤没有影响，但已表明其增加会抑制扩张步骤并降低重新封闭阶段的效率。融合效率受到渗透压和细胞生理状态的极大影响。当细胞贴壁生长且在脉冲处理期间细胞间接触自然存在时，渗透压升高会导致融合指数增加。对于悬浮生长且在脉冲处理后接触的细胞，观察到相反的效果。这些结果初步从渗透压对膜组织和细胞间接触质量的影响方面进行了解释。
