Yu Min, Meifeng Zheng, Ju Sun, Zetong Peng, Zhixian Cao, Xiaohua Huang
Medical College of Acu-Moxi and Rehabilitation, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Department of Rehabilitation Medicine, the Affiliated Panyu Central Hospital of Guangzhou Medical University, Guangzhou 511400, China.
J Tradit Chin Med. 2025 Jun;45(3):610-617. doi: 10.19852/j.cnki.jtcm.20250103.001.
To take Baihui (GV20) and Fengchi (GB20) targeting inflammatory response to regulate migraine as an example to describe a new method for studying the mechanism of stimulating acupoints.
The target information of Baihui (GV20) and Fengchi (GB20) was retrieved, and after intersection with migraine, Kyoto Encyclopedia of Genes and Genomes (KEGG), Reactome, and UniProt Keywords were used for functional enrichment. After selecting the main pathway, rats were selected and nitroglycerin was used for modeling, and the behavioral scores, inflammatory factors, heme oxygenase 1 (HMOX1), protein kinase B (AKT1), signal transducer and activator of transcription 3 (STAT3), phosphorylated extracellular signal-regulated kinase 1/2 (P-ERK1/ERK2) and other states of the rats in the acupuncture, twisting, and electroacupuncture groups were compared.
A total of 135 Baihui (GV20) targets and 27 Fengchi (GB20) targets were collected. A total of 73 target information were obtained after the intersection of these targets in migraine. These 73 targets have three main pathways: hypoxia-inducible factor 1 (HIF-1) signaling pathway, signaling by interleukins and inflammatory response. The main targets in the pathway were verified and found that interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α) and HMOX1, AKT1, STAT3, P-ERK1/ERK2 can be regulated by Baihui (GV20) and Fengchi (GB20).
Baihui (GV20) and Fengchi (GB20) can regulate migraine by regulating inflammatory factors and HMOX1, AKT1, STAT3, P-ERK1/ERK2 and other changes in HIF-1 signaling pathway, Signaling by Interleukins and Inflammatory response pathways. Based on systems biology and network pharmacology, and with the model of "acupoint-target-disease", explore the research methods of systematic acupuncture and moxibustion. We believe this is a usable research direction for exploring the mechanism of acupuncture stimulation.
以针刺百会（GV20）、风池（GB20）靶向炎症反应调控偏头痛为例，阐述一种研究穴位刺激机制的新方法。
检索百会（GV20）和风池（GB20）的靶点信息，与偏头痛进行交集分析后，利用京都基因与基因组百科全书（KEGG）、Reactome和UniProt关键词进行功能富集。选取主要通路后，选用大鼠并用硝酸甘油造模，比较针刺组、捻转组和电针组大鼠的行为学评分、炎症因子、血红素加氧酶1（HMOX1）、蛋白激酶B（AKT1）、信号转导和转录激活因子3（STAT3）、磷酸化细胞外信号调节激酶1/2（P-ERK1/ERK2）等状态。
共收集到135个百会（GV20）靶点和27个风池（GB20）靶点。这些靶点与偏头痛交集后共获得73个靶点信息。这73个靶点有三条主要通路：缺氧诱导因子1（HIF-1）信号通路、白细胞介素信号传导和炎症反应。对通路中的主要靶点进行验证，发现白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）以及HMOX1、AKT1、STAT3、P-ERK1/ERK2均可被百会（GV20）和风池（GB20）调节。
百会（GV20）和风池（GB20）可通过调节炎症因子以及HIF-1信号通路、白细胞介素信号传导和炎症反应通路中的HMOX1、AKT1、STAT3、P-ERK1/ERK2等变化来调控偏头痛。基于系统生物学和网络药理学，以“穴位-靶点-疾病”模型，探索系统针灸的研究方法。我们认为这是探索针刺刺激机制的一个可行的研究方向。
