Sun Yubo, Zhou Yufei, Chen Junquan, Ma Xiaochun, Gong Xiaoqing, Yang Jie, Liu Changhai, Ma Fengwang
State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
Mol Plant Pathol. 2025 Jun;26(6):e70111. doi: 10.1111/mpp.70111.
Plant aquaporins (AQPs), the membrane channels that facilitate the transport of small compounds across plasma membranes or organelle membranes, play a crucial role in regulating various physiological and pathological responses. Here, we report that the apple (Malus domestica) AQP MdPIP2;2 transports HO and enhances the resistance of apple to Colletotrichum fructicola, which causes Glomerella leaf spot (GLS). In response to C. fructicola, MdPIP2;2 is phosphorylated at serine 117 (S117), leading to the enhanced ability of transporting HO. Intriguingly, the apoplastic HO induced by C. fructicola infection is the factor that triggers phosphorylation of MdPIP2;2 at S117, which enhances its ability to activate pattern-triggered immunity (PTI) and confer resistance to GLS. Potentially, MdPIP2;2 and MdPIP2;2 (the phosphomimetic mutant) can be used in crops to improve their disease resistance.
植物水通道蛋白（AQPs）是促进小分子化合物跨质膜或细胞器膜运输的膜通道，在调节各种生理和病理反应中起关键作用。在此，我们报道苹果（Malus domestica）水通道蛋白MdPIP2;2运输H₂O₂并增强苹果对引起炭疽叶枯病（GLS）的胶孢炭疽菌（Colletotrichum fructicola）的抗性。响应胶孢炭疽菌，MdPIP2;2在丝氨酸117（S117）处被磷酸化，导致其运输H₂O₂的能力增强。有趣的是，胶孢炭疽菌感染诱导的质外体H₂O₂是触发MdPIP2;2在S117处磷酸化的因子，这增强了其激活模式触发免疫（PTI）并赋予对炭疽叶枯病抗性的能力。潜在地，MdPIP2;2和MdPIP2;2（磷酸模拟突变体）可用于作物以提高其抗病性。
