Lou Yuhan, Zhang Jiaying, Wu Xirui, Li Jiaxin, Zhou Qihong, Li Wenxiu, Yang Luyuan, Guo Bin
Key Laboratory of Phytochemical R&D of Hunan Province, Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), Institute of Interdisciplinary Studies, Hunan Normal University, Changsha 410081, China.
Anal Methods. 2025 Jul 3;17(26):5412-5422. doi: 10.1039/d5ay00347d.
Natural deep eutectic solvents (NADESs) are emerging green solvents that show great potential in extracting natural products from natural resources. In this study, a third component was introduced into the NADES system, and the screened ternary NADES (choline chloride/fructose/citric acid, 1 : 1 : 2) was combined with ultrasound-enzyme-assisted extraction to extract flavonols from . The main flavonol components, including quercetin, kaempferol and isorhamnetin, were determined by high-performance liquid chromatography and analyzed in comparison with the extraction efficiency of traditional procedures. The results of Fourier transform infrared spectroscopy and Raman spectroscopy showed that the alteration of intermolecular forces in NADESs promoted the formation of hydrogen bonds between them and flavonols, which significantly increased the extraction yields. After optimization by single-factor and Box-Behnken experiments, the ternary NADES extraction yields could reach 0.24 mg g under the optimal conditions of cellulase dosage 1.1%, ultrasonic time 62 min, ultrasonic temperature 61 °C, and water content 60%. The study provides an efficient and environmentally friendly way to extract flavonols from natural medicinal plants, and also elucidates the potential mechanism of the ultrasound-enzyme-assisted ternary NADESs for the improved extraction efficiency.
天然深共熔溶剂（NADESs）是新兴的绿色溶剂，在从自然资源中提取天然产物方面显示出巨大潜力。在本研究中，将第三种成分引入NADES体系，筛选出的三元NADES（氯化胆碱/果糖/柠檬酸，1∶1∶2）与超声酶辅助萃取相结合，用于从……中提取黄酮醇。通过高效液相色谱法测定了包括槲皮素、山奈酚和异鼠李素在内的主要黄酮醇成分，并与传统方法的提取效率进行了比较分析。傅里叶变换红外光谱和拉曼光谱结果表明，NADESs分子间作用力的改变促进了它们与黄酮醇之间氢键的形成，从而显著提高了提取率。通过单因素和Box-Behnken实验优化后，在纤维素酶用量1.1%、超声时间62分钟、超声温度61℃和含水量60%的最佳条件下，三元NADES的提取率可达0.24毫克/克。该研究为从天然药用植物中提取黄酮醇提供了一种高效且环保的方法，同时阐明了超声酶辅助三元NADES提高提取效率的潜在机制。
