Yao Liuyang, Liu Jianing, Pang Yulin, Wang Huimei
State Key Laboratory of Subtropical Silviculture, College of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou 311300, Zhejiang, China; Key Laboratory of Forest Plant Ecology, Ministry of Education, College of Chemistry, Chemistry Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, Heilongjiang, China.
Key Laboratory of Forest Plant Ecology, Ministry of Education, College of Chemistry, Chemistry Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, Heilongjiang, China.
J Chromatogr A. 2025 Aug 30;1757:466166. doi: 10.1016/j.chroma.2025.466166. Epub 2025 Jun 24.
Atractylodes chinensis is a traditional Chinese medicinal plant, which contains lots of volatile oils and phenolic compounds. However, the current researches mainly concentrate on its volatile oils, and only a few studies on phenolic compounds, especially on phenolic compounds extraction in adventitious roots of A. chinensis. Hence, the Ultrasonic-assisted deep eutectic solvent extraction (UAE-DES) was selected to extract phenolic compounds from adventitious roots of A. chinensis. Central composite design (CCD) experiments were employed to find the ideal extraction conditions on the basis of single factor experiments. The results showed that the optimum deep eutectic solvent (DES) was a mixture of choline chloride and ethylene glycol (molar ratio 1:2) with 30 % water. The best extraction conditions for UAE-DES appeared the extraction temperature 55 °C, extraction time 25 min, liquid-solid ratio 32 mL/g, ultrasonic power 300 W. Under these conditions, the extraction yield was 53.63 mg/g, which was 1.37, 1.68 and 1.32 times of ultrasonic-assisted ethanol extraction (UAE-EtOH), ethanol solvent heating extraction (EtOH-SE) and DES solvent heating extraction (DES-SE), respectively. Among these extraction methods, UAE-DES extract showed the strongest antioxidant activity. It was further revealed that Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) analysis identified 10 individual phenolic compounds in the extract by UAE-DES. The contents of six main individual phenolic compounds according to High-Performance Liquid Chromatography (HPLC) analysis were: chlorogenic acid (3.04 mg/g), caffeic acid (1.16 mg/g), neochlorogenic acid (2.00 mg/g), ferulic acid (1.08 mg/g), 7-hydroxycoumarin (0.56 mg/g) and 4,5-dicaffeoylquinic acid (0.60 mg/g). This study suggested that UAE-DES is an efficient and simple method to extract phenolic compounds from A. chinensis adventitious roots, with promising advantage in pharmaceutical industry applications, and the types of phenolic compounds in adventitious roots of A. chinensis were explored to provide theoretical reference for subsequent studies.
白术是一种传统的中药材，含有大量挥发油和酚类化合物。然而，目前的研究主要集中在其挥发油上，对酚类化合物的研究较少，尤其是对白术不定根中酚类化合物的提取研究。因此，选择超声辅助深共熔溶剂萃取法（UAE-DES）从白术不定根中提取酚类化合物。在单因素实验的基础上，采用中心复合设计（CCD）实验来寻找理想的提取条件。结果表明，最佳深共熔溶剂（DES）是氯化胆碱和乙二醇的混合物（摩尔比1:2），含水量为30%。UAE-DES的最佳提取条件为：提取温度55℃，提取时间25分钟，液固比32 mL/g，超声功率300 W。在此条件下，提取率为53.63 mg/g，分别是超声辅助乙醇萃取法（UAE-EtOH）、乙醇溶剂加热萃取法（EtOH-SE）和DES溶剂加热萃取法（DES-SE）的1.37倍、1.68倍和1.32倍。在这些提取方法中，UAE-DES提取物表现出最强的抗氧化活性。进一步的超高效液相色谱-串联质谱（UPLC-MS/MS）分析表明，UAE-DES提取物中鉴定出10种酚类化合物。根据高效液相色谱（HPLC）分析，六种主要酚类化合物的含量分别为：绿原酸（3.04 mg/g）、咖啡酸（1.16 mg/g）、新绿原酸（2.00 mg/g）、阿魏酸（1.08 mg/g）、7-羟基香豆素（0.56 mg/g）和4,5-二咖啡酰奎宁酸（0.60 mg/g）。本研究表明，UAE-DES是一种从白术不定根中提取酚类化合物的高效、简便方法，在制药工业应用中具有广阔的优势，并对白术不定根中酚类化合物的种类进行了探索，为后续研究提供了理论参考。
