来自有序生物聚集体的时间分辨荧光偏振

Time-resolved fluorescence polarization from ordered biological assemblies.

作者信息

Burghardt T P

出版信息

Biophys J. 1985 Oct;48(4):623-31. doi: 10.1016/S0006-3495(85)83818-2.

DOI:10.1016/S0006-3495(85)83818-2

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1329339/

Abstract

We calculate the time dependence of the polarized fluorescence signal from fluorescent-labeled elements of a biological assembly that are rotationally diffusing in an arbitrary three-dimensional angular potential. We have formulated this calculation using the model-independent description of the angular potential wherein the angular potential is described by an expansion in a complete set of orthonormal functions with the expansion coefficients (or order parameters) determined by time-independent methods (Burghardt, T. P., 1984, Biopolymers, 23:2382-2406). We have applied the calculation to fluorescent-labeled myosin cross-bridges in relaxed muscle fibers. In a related paper we describe the experimental observation of the myosin cross-bridges rotationally diffusing in an angular potential (Burghardt, T. P., and N. L. Thompson, 1985, Biochemistry, 24:3731-3735).

摘要

我们计算了生物组件中荧光标记元素的偏振荧光信号随时间的变化，这些元素在任意三维角势中进行旋转扩散。我们使用与模型无关的角势描述来进行此计算，其中角势通过一组完备的正交函数展开来描述，展开系数（或序参量）由与时间无关的方法确定（Burghardt, T. P., 1984, 《生物聚合物》, 23:2382 - 2406）。我们已将该计算应用于松弛肌肉纤维中荧光标记的肌球蛋白横桥。在一篇相关论文中，我们描述了肌球蛋白横桥在角势中旋转扩散的实验观察结果（Burghardt, T. P., 和 N. L. Thompson, 1985, 《生物化学》, 24:3731 - 3735）。

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