El Zaiat Reham Salah, Abouelenin Mai A H, Saleh Amany A, El-Hawy Mahmoud, Ahmedy Iman Aly, Mansour Manal Monir
Department of Clinical Pathology, Faculty of Medicine, Menoufia University, Menoufia Governorate, Egypt.
Medical Biochemistry & Molecular Biology Department, Faculty of Medicine, Menoufia University, Menoufia Governorate, Egypt.
Expert Rev Hematol. 2025 Aug;18(8):689-696. doi: 10.1080/17474086.2025.2522298. Epub 2025 Jun 25.
The pathophysiology of primary immune thrombocytopenia (ITP) is complicated and multifactorial, including platelet antibody formation and T cell imbalance. Emerging evidence has revealed differential miRNA expression in autoimmune disorders, including ITP. Nevertheless, the role of miR-106b-5p, miR-200c-3p, and miR-146a-5p in ITP remains unclear. Herein, we explored the potential role of these miRNAs in pediatric ITP and examined how their plasma levels influenced response to therapy.
Three groups were recruited in this study: newly diagnosed ITP children ( = 25) in group I, chronic ITP children ( = 25) in group II, and normal controls ( = 25) in group III. Plasma levels of miR-106p-5p, miR-200c-3p, and miR-146a-5p were measured by polymerase chain reaction.
MiR-106b-5p and miR-200c-3p were upregulated, whereas miR-146a-5p was downregulated in newly diagnosed and chronic ITP versus controls. MiR-200c-3p and miR-146a-5p were much higher in chronic ITP than newly diagnosed ITP. Lower miR-106b-5p levels were associated with complete response.
MiR-106b-5p and miR-200c-3p were elevated, while miR-146a-5p was suppressed in ITP versus controls. Reduced miR-106b-5p indicated a full response to therapy. These markers may be useful as diagnostic ITP biomarkers. Moreover, miR-106b-5p level can be used to monitor response to therapy and as a predictor for complete response.
原发性免疫性血小板减少症（ITP）的病理生理学复杂且多因素，包括血小板抗体形成和T细胞失衡。新出现的证据显示自身免疫性疾病（包括ITP）中存在差异miRNA表达。然而，miR-106b-5p、miR-200c-3p和miR-146a-5p在ITP中的作用仍不清楚。在此，我们探讨了这些miRNA在儿童ITP中的潜在作用，并研究了它们的血浆水平如何影响治疗反应。
本研究招募了三组：第一组为新诊断的ITP儿童（n = 25），第二组为慢性ITP儿童（n = 25），第三组为正常对照（n = 25）。通过聚合酶链反应测量miR-106p-5p、miR-200c-3p和miR-146a-5p的血浆水平。
与对照组相比，新诊断和慢性ITP中miR-106b-5p和miR-200c-3p上调，而miR-146a-5p下调。慢性ITP中的miR-200c-3p和miR-146a-5p比新诊断的ITP高得多。较低的miR-106b-5p水平与完全缓解相关。
与对照组相比，ITP中miR-106b-5p和miR-200c-3p升高，而miR-146a-5p受到抑制。miR-106b-5p降低表明对治疗有完全反应。这些标志物可能作为ITP的诊断生物标志物有用。此外，miR-106b-5p水平可用于监测治疗反应并作为完全缓解的预测指标。
