斯里兰卡2型糖尿病患者中稳定参考基因的鉴定及微小RNA表达差异：一项横断面研究。

Identification of stable reference genes and differential miRNA expression in Sri Lankan type 2 diabetes mellitus patients: a cross-sectional study.

作者信息

Palihaderu Palihaderu Arachchige Dineth Supasan, Mendis Balapuwaduge Isuru Layan Madusanka, Premarathne Jayasekara Mudiyanselage Krishanthi Jayarukshi Kumari, Dias Wajjakkara Kankanamlage Ruwin, Yeap Swee Keong, Ho Wan Yong, Dissanayake Arosha Sampath, Rajapakse Iyanthimala Harshini, Karunanayake Panduka, Senarath Upul, Satharasinghe Dilan Amila

机构信息

Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka.

Department of Livestock and Avian Sciences, Faculty of Livestock, Fisheries, and Nutrition, Wayamba University of Sri Lanka, Makandura, Gonawila, Sri Lanka.

出版信息

Front Endocrinol (Lausanne). 2025 Jun 12;16:1554827. doi: 10.3389/fendo.2025.1554827. eCollection 2025.

DOI:10.3389/fendo.2025.1554827

PMID:40575262

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12197912/

Abstract

INTRODUCTION

Type 2 Diabetes mellitus is a major global health concern. MicroRNA plays an important role in regulating pancreatic beta cells as well as peripheral insulin signaling. This study aimed to identify reference microRNA s in type 2 Diabetes mellitus plasma and validate two target microRNAs among a Sri Lankan population with type 2 diabetes mellitus.

METHODS

This is a cross-sectional experiment. A total of fifty-three (N = 53) non-hemolyzed plasma samples from individuals with type 2 diabetes mellitus were selected to evaluate stability, in comparison to thirty-eight (N = 38) normoglycemic non-hemolyzed plasma samples. Initially, the stability of four candidate reference microRNAs (hsa-miR-16-5p, hsa-miR-425-5p, hsa-miR-191-5p, and hsa-miR-22-5p) was assessed. Stability was analyzed using the geNorm and BestKeeper algorithms. The relative expression changes of hsa-miR-29a-3p and hsa-miR-375-3p in the plasma of the same samples were evaluated using the validated reference microRNAs. The selected regulatory microRNAs were directly linked with type 2 diabetes mellitus pathogenesis and proved to be upregulated in type 2 diabetes mellitus plasma and serum.

RESULTS AND DISCUSSION

The expressions of miR-16-5p and miR-191-5p were not stable between the two groups, miR-22-5p and miR-425-5p levels were found to be stable. A significant upregulation of hsa-miR-29a-3p and hsa-miR-375-3p was observed in type 2 diabetes mellitus patients compared to normoglycemic individuals (p ≤ 0.05). This was the first study to claim hsa-miR-425-5p and hsa-miR-22-5p as stably expressed reference microRNAs in type 2 diabetes mellitus patients. Sri Lankan type 2 diabetic patients also had increased hsa-miR-29a-3p and hsa-miR-375-3p levels. However, large and well-matched sample studies were suggested to ensure that these microRNAs can be used as type 2 diabetes diagnostic markers in Sri Lanka.

摘要

引言

2型糖尿病是全球主要的健康问题。微小RNA在调节胰岛β细胞以及外周胰岛素信号传导中发挥重要作用。本研究旨在鉴定2型糖尿病血浆中的参考微小RNA，并在斯里兰卡2型糖尿病患者群体中验证两个靶标微小RNA。

方法

这是一项横断面实验。与38份非溶血正常血糖血浆样本相比，共选取53份2型糖尿病患者的非溶血血浆样本评估稳定性。最初，评估了四种候选参考微小RNA（hsa-miR-16-5p、hsa-miR-425-5p、hsa-miR-191-5p和hsa-miR-22-5p）的稳定性。使用geNorm和BestKeeper算法分析稳定性。使用经过验证的参考微小RNA评估相同样本血浆中hsa-miR-29a-3p和hsa-miR-375-3p的相对表达变化。所选的调节性微小RNA与2型糖尿病发病机制直接相关，并被证明在2型糖尿病血浆和血清中上调。

结果与讨论

两组之间miR-16-5p和miR-191-5p的表达不稳定，发现miR-22-5p和miR-425-5p水平稳定。与正常血糖个体相比，2型糖尿病患者中观察到hsa-miR-29a-3p和hsa-miR-375-3p显著上调（p≤0.05）。这是第一项声称hsa-miR-425-5p和hsa-miR-22-5p为2型糖尿病患者中稳定表达的参考微小RNA的研究。斯里兰卡2型糖尿病患者的hsa-miR-29a-3p和hsa-miR-375-3p水平也有所升高。然而，建议进行大规模且匹配良好的样本研究，以确保这些微小RNA可作为斯里兰卡2型糖尿病的诊断标志物。