Hu Yonghua, Su Weichao, Wang Baoxuan, Yang Xiaoyu, Ke Lina, Shi Yan, Wang Yuleyue, Wang Qin
School of Biological Science and Biotechnology, Minnan Normal University, Zhangzhou , Fujian, 363000, China.
School of Life Sciences, Xiamen University, Xiamen, Fujian, 361102, China.
Appl Biochem Biotechnol. 2025 Jun 17. doi: 10.1007/s12010-025-05296-z.
Shark skin peptides, a kind of substance with a variety of biological activities, were shown to inhibit the activity of mushroom tyrosinase. This study was performed to clarify their inhibitory effects on melanin synthesis in B16F10 melanocytes and in vivo zebrafish models. In order to elucidate the inhibitory effects of shark skin peptides on B16F10 melanocytes, cell viability, morphologic changes, intracellular tyrosinase, and the melanogenesis-related proteins were determined. The results showed that the peptides were not cytotoxic to B16F10, and the morphology of the cells did not change significantly at the detected concentration. The intracellular melanin content and tyrosinase activity of B16F10 cells decreased in a concentration-dependent manner after the peptide treatment. The suppressive activity of the peptides on melanin synthesis is linked to the down-regulation of the important proteins involved in melanogenesis, such as tyrosinase, tyrosinase-related protein-1 (TRP1), and microphthalmia-associated transcription factor (MITF). The effects of shark skin peptides on melanin synthesis in zebrafish embryos showed that the peptides inhibit the production of melanin in embryos, and the melanin-related proteins were also detected as down-regulated. The results from ROS analysis suggested that the peptides are also capable of eliminating reactive oxygen species within the cells. In conclusion, the shark skin peptides could inhibit melanin synthesis, indicating that peptides may become additives for cosmetics potentially.
鲨鱼皮肽是一种具有多种生物活性的物质，已被证明能抑制蘑菇酪氨酸酶的活性。本研究旨在阐明其对B16F10黑素细胞和体内斑马鱼模型中黑色素合成的抑制作用。为了阐明鲨鱼皮肽对B16F10黑素细胞的抑制作用，测定了细胞活力、形态变化、细胞内酪氨酸酶以及与黑色素生成相关的蛋白质。结果表明，这些肽对B16F10细胞无细胞毒性，在检测浓度下细胞形态无明显变化。肽处理后，B16F10细胞内黑色素含量和酪氨酸酶活性呈浓度依赖性降低。这些肽对黑色素合成的抑制活性与黑色素生成过程中重要蛋白质的下调有关，如酪氨酸酶、酪氨酸酶相关蛋白-1（TRP1）和小眼畸形相关转录因子（MITF）。鲨鱼皮肽对斑马鱼胚胎黑色素合成的影响表明，这些肽能抑制胚胎中黑色素的产生，并且与黑色素相关的蛋白质也被检测到表达下调。活性氧分析结果表明，这些肽还能够清除细胞内的活性氧。总之，鲨鱼皮肽可以抑制黑色素合成，这表明这些肽可能成为潜在的化妆品添加剂。
