Shim Joong-Hyun
Department of Cosmetic Science, Semyung University, Science & Engineering Bldg/Room 313, Chungbuk 390-711, Korea.
Molecules. 2021 Apr 26;26(9):2526. doi: 10.3390/molecules26092526.
This study was performed to clarify the inhibitory effects of cycloheterophyllin on melanin synthesis. In order to elucidate the inhibitory effects of cycloheterophyllin on the B16F10 cell line, cell viability, messenger ribonucleic acid (mRNA) expressions, tyrosinase activity assay, and melanin production assay were measured. The effects of cycloheterophyllin on tyrosinase-related protein 1 ()//tyrosinase ()/microphthalmia-associated transcription factor () mRNA expressions and melanin content were determined. Quantitative real-time RT-PCR showed that cycloheterophyllin decreased the mRNA expression level of genes and melanin production contents than α-MSH-treated B16F10 cells. The tyrosinase activity assay revealed that cycloheterophyllin decreased the melanin production in the B16F10 cells. These data show that cycloheterophyllin increases the whitening effects in the B16F10 cells; thus, cycloheterophyllin is a potent ingredient for skin whitening. Thus, further research on the mechanism of action of cycloheterophyllin for the development of functional materials should be investigated.
本研究旨在阐明环叶异椴素对黑色素合成的抑制作用。为了阐明环叶异椴素对B16F10细胞系的抑制作用，检测了细胞活力、信使核糖核酸（mRNA）表达、酪氨酸酶活性测定和黑色素生成测定。测定了环叶异椴素对酪氨酸酶相关蛋白1（TRP-1）/酪氨酸酶（TYR）/小眼畸形相关转录因子（MITF）mRNA表达和黑色素含量的影响。定量实时RT-PCR显示，与α-MSH处理的B16F10细胞相比，环叶异椴素降低了相关基因的mRNA表达水平和黑色素生成含量。酪氨酸酶活性测定表明，环叶异椴素降低了B16F10细胞中的黑色素生成。这些数据表明，环叶异椴素增强了B16F10细胞的美白效果；因此，环叶异椴素是一种有效的皮肤美白成分。因此，应进一步研究环叶异椴素的作用机制，以开发功能性材料。
