State of the art indicators for imaging purinergic dynamics in vitro and in vivo.

Purinergic neurotransmission, a dynamic signaling system using adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine (ADO), uridine diphosphate (UDP), and others, plays a crucial role in brain function. Purinergic signaling is involved in regulating synaptic communication to influence sleep and neuroprotection; malfunction of purinergic signaling contributes to various neurological disorders like pain, epilepsy, and depression. Effective detection methods are crucial for a comprehensive understanding of the multifaceted roles of purinergic signaling in the brain. This review sheds light on advancements in fluorescent indicators, a powerful toolkit for visualizing purinergic activities in living animals. We explore the diverse applications of these indicators in studying purinergic transmission both in health and in diseases. Despite their current strengths, we emphasize the need for continuous development of fluorescent indicators to achieve an even more comprehensive, specific, and quantitative detection of purinergic signaling.