Das Sony Moni, Gaur Aditi, Deep Shashank
Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.
Langmuir. 2025 Jul 1;41(25):15828-15840. doi: 10.1021/acs.langmuir.5c00537. Epub 2025 Jun 17.
Cataract, one of the leading causes of vision loss, is primarily caused by the aggregation of crystallin proteins in the eye lens. This research work examines how oxidative stress, triggered by HO, influences the aggregation of human γD-crystallin. Techniques such as turbidity assay, thioflavin T (ThT) assay, circular dichroism (CD) spectroscopy, 1-anilinonapthalene-8-sulfonate (ANS) binding assay, Fourier transform infrared (FTIR) study, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis, and transmission electron microscopy (TEM) confirm the aggregation of protein in the presence of HO-forming fibrils. Polyphenols, resveratrol and quercetin, are observed not only to inhibit protein aggregation caused by HO but can be used to break the fibrils. A nanodelivery system prepared from chitosan (CS) and polylactic--glycolic acid (PLGA) was used to encapsulate the polyphenols. TEM, dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), thermogravimetric analysis (TGA), and X-ray diffraction (XRD) were used to characterize the nanoparticles (NPs). NPs are 100 nm in size, crystalline in nature, and nontoxic to cells. They improve the bioavailability of polyphenols. Multiple approaches were used to confirm that CS-PLGA NPs loaded with quercetin and resveratrol effectively prevented γD-crystallin aggregation.
白内障是导致视力丧失的主要原因之一，主要由眼晶状体中晶状体蛋白的聚集引起。这项研究工作考察了由羟基引发的氧化应激如何影响人γD-晶状体蛋白的聚集。诸如浊度测定、硫黄素T（ThT）测定、圆二色性（CD）光谱、1-苯胺基萘-8-磺酸盐（ANS）结合测定、傅里叶变换红外（FTIR）研究、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析以及透射电子显微镜（TEM）等技术证实了在羟基存在下蛋白质形成原纤维的聚集。观察到多酚、白藜芦醇和槲皮素不仅能抑制由羟基引起的蛋白质聚集，还可用于破坏原纤维。由壳聚糖（CS）和聚乳酸-乙醇酸（PLGA）制备的纳米递送系统用于包封多酚。利用TEM、动态光散射（DLS）、纳米颗粒跟踪分析（NTA）、热重分析（TGA）和X射线衍射（XRD）对纳米颗粒（NPs）进行表征。NPs尺寸为100 nm，本质上是晶体，对细胞无毒。它们提高了多酚的生物利用度。采用多种方法证实负载槲皮素和白藜芦醇的CS-PLGA NPs能有效防止γD-晶状体蛋白聚集。
