Watson R E, Siegel H E, Siegel A
Brain Res. 1985 Oct 28;346(1):89-107. doi: 10.1016/0006-8993(85)91098-4.
The [14C]2-deoxyglucose (2-DG) metabolic mapping technique has been used to identify the regions responding with an augmented rate of metabolism following focal electrical stimulation of various sites within the lateral septal nucleus and medial septal nucleus/diagonal band (MSN/DB) complex in the rat. Since 2-DG uptake has been correlated with rates of functional activity, it was the intention of this study to suggest the anatomical substrates underlying various physiological and behavioral responses elicited by stimulation of the septal area. The results show that stimulation of any region within the lateral septal nucleus produced a profound bilateral activation of both the lateral septal nucleus, as well as the hippocampal formation. While stimulation of a number of different fiber systems associated with the lateral septum could contribute to the observed pattern of labeling, the data suggest that, functionally, a major consequence of such stimulation is the antidromic activation of CA3----lateral septum fibers to axonal branch points, beyond which, orthodromic propagation of the impulse produces activation in CA3 target regions, including subfields CA1 and CA3, as well as the lateral septal nucleus, bilaterally. In addition, regions typically manifesting metabolic activation following stimulation of the lateral septal nucleus included the ipsilateral diagonal band of Broca, nucleus accumbens, lateral preoptic area and lateral hypothalamus, posteriorly, and the prelimbic cortex, anteriorly. Occasionally, target regions of the postcommissural fornix, including the medial mammillary nucleus and anterior thalamic nuclei were also activated following stimulation of the lateral septal nucleus. In contrast to the widespread pattern of activation resulting from stimulation of the lateral septal nucleus, stimulation of the MSN/DB complex produced activation which was largely confined to the medial forebrain bundle. In a final phase of the experiment, afterdischarge activity was elicited by sodium penicillin injection into the lateral septal nucleus. Such treatment produced more widespread 2-DG uptake, including more extensive activation within the lateral septal nucleus, hippocampal formation, amygdala, and thalamus. Additionally, the prefrontal cortex and temporal neocortex were activated.
[14C]2-脱氧葡萄糖(2-DG)代谢图谱技术已被用于识别大鼠外侧隔核和内侧隔核/斜角带(MSN/DB)复合体中不同部位受到局部电刺激后代谢率增加的反应区域。由于2-DG摄取与功能活动速率相关，本研究旨在揭示刺激隔区引发的各种生理和行为反应背后的解剖学基础。结果表明，刺激外侧隔核内的任何区域都会导致外侧隔核以及海马结构出现双侧显著激活。虽然与外侧隔相关的许多不同纤维系统的刺激可能导致观察到的标记模式，但数据表明，从功能上讲，这种刺激的一个主要后果是CA3→外侧隔纤维向轴突分支点的逆向激活，在此之后，冲动的顺向传播在双侧CA3靶区域产生激活，包括CA1和CA3亚区以及外侧隔核。此外，外侧隔核刺激后通常表现出代谢激活的区域包括同侧的Broca斜角带、伏隔核、外侧视前区和下丘脑后部，以及前部的前边缘皮层。偶尔，在外侧隔核刺激后，连合后穹窿的靶区域，包括内侧乳头体核和前丘脑核也会被激活。与外侧隔核刺激产生的广泛激活模式不同，MSN/DB复合体的刺激产生的激活主要局限于内侧前脑束。在实验的最后阶段，通过向外侧隔核注射青霉素钠引发后放电活动。这种处理导致更广泛的2-DG摄取，包括外侧隔核、海马结构、杏仁核和丘脑内更广泛的激活。此外，前额叶皮层和颞叶新皮层也被激活。
