Mosaad Habiba, Elekhnawy Engy, Negm Walaa A, Younis Salwa S, El-Aasr Mona
Gharbiya STEM High School, Tanta, Egypt.
Microbiology and Immunology Department, Faculty of Pharmacy, Tanta University, Tanta, Egypt.
Curr Microbiol. 2025 Jun 18;82(8):338. doi: 10.1007/s00284-025-04316-9.
In light of the propagation of resistance amongst bacteria, many infections are currently difficult to treat, with extremely high death and morbidity rates. Thus, we investigated the effect of using a combination of amoxicillin and broccoli ethanolic extract against Staphylococcus aureus infections. Broccoli or Brassica oleracea active metabolites' were to be identified and quantified using high-performance liquid chromatography (HPLC). The major detected compounds were chlorogenic acid and gallic acid. In vitro, the combination of amoxicillin with broccoli revealed fractional inhibitory concentration index of the amoxicillin-broccoli combination was less than 0.5 in 50% of the isolates, which indicates a synergetic potential. The in vivo study revealed a notable lessening in the bacterial count present in the examined tissues in the employed systemic infection model. The studied tissues were kidney, liver, and spleen. Moreover, the histological characteristics were found to have a remarkable enhancement in the combination-treated group. Thus, future clinical reports have to be employed to explicate the usefulness of such a combination in the clinical practice.
鉴于细菌耐药性的传播，目前许多感染难以治疗，死亡率和发病率极高。因此，我们研究了阿莫西林与西兰花乙醇提取物联合使用对金黄色葡萄球菌感染的影响。将使用高效液相色谱法（HPLC）对西兰花或甘蓝的活性代谢产物进行鉴定和定量。检测到的主要化合物是绿原酸和没食子酸。在体外，阿莫西林与西兰花的组合显示，在50%的分离株中，阿莫西林 - 西兰花组合的分数抑制浓度指数小于0.5，这表明具有协同潜力。体内研究显示，在所采用的全身感染模型中，受检组织中的细菌数量显著减少。研究的组织是肾脏、肝脏和脾脏。此外，发现联合治疗组的组织学特征有显著改善。因此，未来必须采用临床报告来阐明这种组合在临床实践中的有效性。
