Wendt Alexander S, Brannelly Laura
Department of Veterinary Sciences, The University of Melbourne Faculty of Science, Werribee, Victoria, 3030, Australia.
F1000Res. 2025 May 23;14:514. doi: 10.12688/f1000research.163701.1. eCollection 2025.
The alpine tree frog ( ) is a threatened species found only above 1,200 meters within the Australian Alps. This species' distribution has been severely limited due to the pathogentic amphibian chytrid fungus, and current populations persist by recruitment. Here, we provide the first publicly available genome for the genus. We used PacBio HiFi reads as well as Omni-C scaffolding data to construct a high-quality genome. We also generated a reproduction focused transcriptome from brain, liver, and gonad tissues. The genome was 2.77 Gb in length and consisted of 962 contigs with a contig N50 of 37.2 Mb and an L50 of 19. This study provides the first publicly available reference genome for the genus to assist in conservation and reproduction focused works in amphibian management.
高山树蛙（ ）是一种濒危物种，仅在澳大利亚阿尔卑斯山脉海拔1200米以上的地区被发现。由于致病性两栖壶菌，该物种的分布受到严重限制，目前的种群通过繁殖得以维持。在这里，我们提供了该属第一个公开可用的基因组。我们使用PacBio HiFi reads以及Omni-C支架数据构建了一个高质量的基因组。我们还从脑、肝和性腺组织中生成了一个以繁殖为重点的转录组。该基因组长度为2.77 Gb，由962个重叠群组成，重叠群N50为37.2 Mb，L50为19。这项研究提供了该属第一个公开可用的参考基因组，以协助两栖动物管理中以保护和繁殖为重点的工作。
