Kosch Tiffany A, Berger Lee, Crawford Andrew J, Gilbert Deon, Goodall Damian, Hunter David, McFadden Michael, Abueg Linelle, Formenti Giulio, Collins Joanna, Tilley Tatiana, Jain Nivesh, O'Toole Brian, Whelan Conor, Cooke Ira, Muffato Matthieu, Yates Bethan, Fedrigo Olivier, Balacco Jennifer, Jarvis Erich D, Skerratt Lee F
Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Melbourne, Victoria, Australia.
Departamento de Ciencias Biológicas, Universidad de Los Andes, Bogotá, Colombia.
Wellcome Open Res. 2025 Apr 30;10:228. doi: 10.12688/wellcomeopenres.23820.1. eCollection 2025.
The Southern Corroboree frog ( ; Anura; Myobatrachidae) is a Critically Endangered amphibian, according to the IUCN, and is endemic to the Snowy Mountains region of Kosciuszko National Park in New South Wales, Australia. This species has been driven to functional extinction by the introduction of the fungal disease, chytridiomycosis. Here we provide the first reference genome for . Using PacBio HiFi sequencing, Arima Hi-C, and Bionano optical mapping, we produced a chromosome-level genome assembly. Additionally, we generated a reference transcriptome based on multiple tissues from both male and female individuals to support genome annotation. The resulting genome spans 8.87 Gb across 12 chromosomes, with a contig N50 of 6.8 Mb. This research provides a phased, annotated genome assembly along with transcriptomic resources to facilitate future conservation genomic studies of . Furthermore, the genome offers an invaluable resource for taxonomic and evolutionary research, particularly given the nearest available chromosome-level reference genome is from , a species that last shared a common ancestor with 80 million years ago.
南部灌丛蛙（；无尾目；短头蟾科）是一种极度濒危的两栖动物，根据国际自然保护联盟（IUCN）的标准，它是澳大利亚新南威尔士州科西阿斯科山国家公园雪山地区的特有物种。由于真菌疾病壶菌病的引入，该物种已功能性灭绝。在此，我们提供了的首个参考基因组。利用PacBio HiFi测序、Arima Hi-C和Bionano光学图谱技术，我们构建了一个染色体水平的基因组组装。此外，我们基于雄性和雌性个体的多个组织生成了一个参考转录组，以支持基因组注释。最终得到的基因组跨越12条染色体，大小为8.87 Gb，重叠群N50为6.8 Mb。这项研究提供了一个分阶段注释的基因组组装以及转录组资源，以促进未来对的保护基因组学研究。此外，该基因组为分类学和进化研究提供了宝贵资源，特别是考虑到最近可用的染色体水平参考基因组来自，而该物种与在8000万年前最后拥有共同祖先。
