Zhang Mengping, Pan Hui, Zeng Yafen, Meng Xiao, Chen Wenwen, Jin Meng, Shao Hua, Wei Haiyan, Wang Cuijuan
Physical and Chemical Laboratory, Shandong Academy of Occupational Health and Occupational Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, 250000, China.
Curr Res Food Sci. 2025 Jun 3;10:101106. doi: 10.1016/j.crfs.2025.101106. eCollection 2025.
Fenitrothion and methyl parathion residues pose significant public health risks. Efficiently extracting and real-time detecting pesticide residues in complex matrices remains challenging. This study presents an ultrafast enrichment and SERS detection approach, achieving rapid analysis of fenitrothion and methyl parathion in vegetable and fruit juices using a dual-functionalized microneedle. A point-of-use SERS-active microneedle was developed with rough silver covered with an Au layer for ultrafast electro-enhanced microextraction (EEM) and sensitive SERS response toward real-time monitoring. Applying a negative electric potential to the dual-functionalized microneedle, pesticide enrichment was accelerated, achieving a tenfold increase in efficiency over traditional static strategy. The EEM-SERS process can be completed in 5 min, with strong correlations noted between SERS intensity and pesticide concentrations ( = 0.940-0.976). The detection limits for fenitrothion and methyl parathion were 1.08-1.88 ppb in celery and orange juice, demonstrating the method's effectiveness for quick in situ pesticide residue detection.
杀螟硫磷和甲基对硫磷残留对公众健康构成重大风险。在复杂基质中高效提取和实时检测农药残留仍然具有挑战性。本研究提出了一种超快速富集和表面增强拉曼光谱（SERS）检测方法，使用双功能微针实现了对蔬菜汁和果汁中杀螟硫磷和甲基对硫磷的快速分析。开发了一种即用型SERS活性微针，其粗糙银表面覆盖有金层，用于超快速电增强微萃取（EEM）以及对实时监测的灵敏SERS响应。对双功能微针施加负电势可加速农药富集，效率比传统静态策略提高了十倍。EEM-SERS过程可在5分钟内完成，SERS强度与农药浓度之间存在强相关性（r = 0.940 - 0.976）。芹菜汁和橙汁中杀螟硫磷和甲基对硫磷的检测限为1.08 - 1.88 ppb，证明了该方法用于快速原位农药残留检测的有效性。
