Superhydrophobic nanocellulose-based self-assembled flexible SERS substrates for pesticide detection.

Flexible surface-enhanced Raman scattering (SERS) substrates that provide simple sampling are helpful for the on-site detection of explosive contamination, pesticide residues on food surfaces, and water pollution in public spaces. Using superhydrophobic nanocellulose-based film as the support, 2D flexible SERS substrates that integrated sampling, enrichment, and detection were successfully fabricated via the solvent-induced evaporation method. This approach enabled the co-loading of two plasmonic nanoparticles with different sizes and shapes. A uniform and dense distribution of two-dimensional "hot spots" was created by the plasmonic nanoparticles' self-assembly on the hydrophobic substrate. By adjusting the loading ratio of Au-core/Ag-shell nanocubes and gold nanospheres, their synergistic effect optimized the "hot spots" structure and significantly increased the SERS signal intensity. Additionally, the hydrophobic property of the substrate allowed the target analytes to be concentrated throughout the drying process, significantly increasing the sensitivity of SERS detection. This flexible substrate can sensitively and accurately detect the pesticide residues of phosphorus and methyl parathion on apple peel with the detection limit of 10 g/L and relative standard deviation (RSD) less than 10 %. The high-performance SERS substrate has great potential for in-situ detection applications such as food safety and environmental monitoring.