Shehabeldin Ahmed M, Salama Maha S, Omar Mohamed E A, AbdEl-Rafaa Liza A, Ashour Mohey A, Hassan Mahmoud A E, El-Shereif Abdelghany A, Abdelmegeid Mohamed, Shukry Mustafa, Elolimy Ahmed A
Animal Production Research Institute (APRI), Agricultural Research Center (ARC), Giza, Egypt.
Animal Reproduction Research Institute (ARRI), Agricultural Research Center (ARC), Giza, Egypt.
Front Vet Sci. 2025 Jun 3;12:1607674. doi: 10.3389/fvets.2025.1607674. eCollection 2025.
The present study evaluated the efficacy of pregnant mare serum gonadotropin (PMSG) and PMSG encapsulated in chitosan-tripolyphosphate nanoparticles in enhancing reproductive performance in short-term progesterone estrus-synchronized Ossimi ewes.
Seventy-five healthy ewes were randomly assigned to three groups ( = 25 per group). Group 1 (representing current standard practice) received 25 mg progesterone acetate for 7 days, 600 IU PMSG on day 6, and 250 μg prostaglandin F (PGF) on day 7. Group 2 followed the same regimen as Group 1, except for administering 300 IU of PMSG-loaded chitosan-tripolyphosphate nanoparticles on day 6, followed by an intramuscular injection of 250 μg PGF on day 7. Group 3 received 150 IU of PMSG-loaded chitosan-tripolyphosphate nanoparticles on day 6 and 250 μg PGF on day 7. Estrus detection occurred between days 7 and 11, with a gonadotropin-releasing hormone (GnRH) injection at breeding.
Group 1 had a significantly shorter onset of estrus (54.40 ± 4.50 h; < 0.05) compared to Group 2 (71.60 ± 0.51 h) and Group 3 (72.20 ± 4.81 h). Pregnancy and lambing rates were highest in Group 2 (100%; < 0.05), and Group 2 produced more fetuses (40) than Group 1 (30) and Group 3 (25). Fecundity was also highest in Group 2 (160%; < 0.05). Follicular diameter was greater in Group 2 on day 9, although the number of large follicles was similar across groups. The number of corpora lutea significantly increased on day 7 compared to day 0 in all groups. Progesterone levels peaked on day 7 and declined by day 9 across all groups. These results suggest that administering 300 IU of PMSG encapsulated in chitosan-tripolyphosphate nanoparticles can enhance reproductive performance more effectively than conventional PMSG, offering a promising strategy to improve fertility in short-term progesterone-synchronized ewes.
本研究评估了孕马血清促性腺激素（PMSG）以及包裹在壳聚糖-三聚磷酸纳米颗粒中的PMSG对短期孕酮发情同步化的奥西米母羊繁殖性能的增强作用。
75只健康母羊随机分为三组（每组n = 25只）。第1组（代表当前标准做法）接受25 mg醋酸孕酮注射7天，在第6天注射600 IU PMSG，在第7天注射250 μg前列腺素F（PGF）。第2组遵循与第1组相同的方案，但在第6天给予300 IU负载PMSG的壳聚糖-三聚磷酸纳米颗粒，然后在第7天肌肉注射250 μg PGF。第3组在第6天接受150 IU负载PMSG的壳聚糖-三聚磷酸纳米颗粒，并在第7天接受250 μg PGF。在第7至11天进行发情检测，配种时注射促性腺激素释放激素（GnRH）。
与第2组（71.60 ± 0.51小时）和第3组（72.20 ± 4.81小时）相比，第1组的发情开始时间显著更短（54.40 ± 4.50小时；P < 0.05）。第2组的妊娠率和产羔率最高（100%；P < 0.05），并且第2组产生的胎儿（40只）比第1组（30只）和第3组（25只）更多。第2组的繁殖力也最高（160%；P < 0.05）。在第9天，第2组的卵泡直径更大，尽管各组之间大卵泡的数量相似。与第0天相比，所有组在第7天的黄体数量显著增加。所有组的孕酮水平在第7天达到峰值，并在第9天下降。这些结果表明，给予300 IU包裹在壳聚糖-三聚磷酸纳米颗粒中的PMSG比传统PMSG能更有效地提高繁殖性能，为提高短期孕酮同步化母羊的生育力提供了一种有前景的策略。
