Prognostic significance of and its methylation in lung adenocarcinoma.

BACKGROUND

Interleukin-11 receptor alpha () involves in numerous biological processes of malignant tumor. Nevertheless, the precise role of in lung adenocarcinoma (LUAD) remains unknown. This research aimed to elucidate the differential expression, methylation, prognosis significance and immune infiltration of in LUAD.

METHODS

Using the University of Alabama at Birmingham Cancer Data Analysis Portal (UALCAN), The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), ONCOMINE, Gene Expression Profiling Interactive Analysis (GEPIA), and Human Protein Atlas (HPA) databases, the varied expression of in LUAD was confirmed. The level of methylation was investigated by UALCAN database. Spearman method was utilized to analyze the relevance between expression and its methylation. The prognostic significance of methylation was assessed using the Kaplan-Meier method. Furthermore, a meta-analysis based on TCGA and GEO databases was executed to judge the prognostic significance of in LUAD. Immune infiltration was evaluated by Gene set enrichment analysis (GSEA) analysis, single-sample gene set enrichment analysis (ssGSEA) analysis and Tumor Immune Estimation Resource (TIMER) database.

RESULTS

Our research demonstrated that expression was markedly diminished in LUAD. The low expression of was tightly linked with stage, T stage, N stage and cancer status. gene was inversely regulated by hypermethylation, resulting in its low expression in LUAD. The hypermethylation of implied poor prognosis in LUAD. Moreover, a meta-analysis of 2,964 LUAD patients from 23 studies demonstrated that low expression was positively associated with dismal overall survival (OS). Subsequently, we demonstrated that low expression of was closely linked to diverse immune cells, especially B cells.

CONCLUSIONS

Our work demonstrates that might be an underlying prognostic biomarker and an innovative molecular therapeutic target for individuals with LUAD.