Jia Yifan, Wang Jianwei, Lin Xiaojie, Liang Taibo, Dai Huaxin, Wu Baojian, Yang Mengmeng, Zhang Yanling, Li Ruifang
Zhengzhou Key Laboratory of Functional Molecules for Biomedical Research, Henan University of Technology, Zhengzhou, Henan, China.
College of Biological Engineering, Henan University of Technology, Zhengzhou Henan, China.
Front Plant Sci. 2025 Jun 3;16:1588888. doi: 10.3389/fpls.2025.1588888. eCollection 2025.
Current research on tobacco aroma predominantly focuses on single-omics approaches. In this study, we conducted a comprehensive investigation of the relationships between tobacco metabolite profiles, microbial communities, and aroma characteristics. Untargeted metabolomics and metagenomic analyses were performed on flue-cured upper tobacco leaves to compare light aromatic tobacco (LAT) and strong aromatic tobacco (SAT). The results showed that sugar metabolite levels in LAT were significantly higher than those in SAT, whereas levels of specific acids and amino acid metabolites in SAT exceeded those in LAT. Redundancy analysis (RDA) and metabolomic correlation analyses indicated that the genera and may promote sugar metabolite accumulation, while potentially regulates both sugar and acid metabolites. In contrast, and were associated with acid and amino acid metabolism, with additionally exhibiting inhibitory effects on sugar metabolism. Metagenomic analysis revealed that , , and were abundant in LAT, whereas and dominated in SAT. Notably, the bidirectional regulation of aromatic metabolites by microbial genera such as highlights the universality of plant-microbe interactions in shaping metabolic networks-a mechanism potentially applicable to other crop systems. These findings reveal conserved microbial functional traits (e.g., metabolic pathway modulation) that may drive plant phenotypic differentiation beyond tobacco, offering insights into microbiome-mediated crop quality improvement. The results provide theoretical guidance for tobacco aging and aroma regulation and underscore the broader significance of microbial community engineering in agriculture for manipulating plant metabolic outputs.
目前对烟草香气的研究主要集中在单一组学方法上。在本研究中，我们对烟草代谢物谱、微生物群落和香气特征之间的关系进行了全面调查。对烤烟上部烟叶进行了非靶向代谢组学和宏基因组分析，以比较清香型烟草（LAT）和浓香型烟草（SAT）。结果表明，LAT中的糖代谢物水平显著高于SAT，而SAT中特定酸和氨基酸代谢物的水平超过LAT。冗余分析（RDA）和代谢组学相关性分析表明，[具体属名1]和[具体属名2]可能促进糖代谢物积累，而[具体属名3]可能同时调节糖和酸代谢物。相比之下，[具体属名4]和[具体属名5]与酸和氨基酸代谢相关，[具体属名5]还对糖代谢表现出抑制作用。宏基因组分析显示，[具体属名6]、[具体属名7]和[具体属名8]在LAT中含量丰富，而[具体属名9]和[具体属名10]在SAT中占主导地位。值得注意的是，[具体属名11]等微生物属对芳香代谢物的双向调节突出了植物-微生物相互作用在塑造代谢网络中的普遍性——这一机制可能适用于其他作物系统。这些发现揭示了保守的微生物功能特征（如代谢途径调节），这些特征可能驱动烟草以外的植物表型分化，为微生物组介导的作物品质改良提供了见解。研究结果为烟草陈化和香气调控提供了理论指导，并强调了农业中微生物群落工程在操纵植物代谢产物方面的更广泛意义。
