Chandel Neha Singh, Singh H B, Vaishnav Anukool
Department of Biotechnology, GLA University, Mathura, Uttar Pradesh, India.
Front Plant Sci. 2025 Mar 27;16:1577521. doi: 10.3389/fpls.2025.1577521. eCollection 2025.
Plants release a wealth of metabolites into the rhizosphere that can influence the composition and activity of microbial communities. These communities, in turn, can affect the growth and metabolism of the host plant. The connection between medicinal plant and its associated microbes has been suggested, yet the mechanisms underlying selection of indigenous microbes, and their biological function in medicinal plants are largely unknown. In this study, we investigated how the plants select its rhizosphere bacteria and examined their functional roles in relation to plant benefit. We utilized two native plant growth promoting rhizobacterial (PGPR) strains of : sp. GLAU-BT2 and sp. GLAU-BT16, as either single or consortium inoculants for plant growth experiment. We analyzed non-targeted root metabolites in the presence of both single and consortium bacterial inoculants and confirmed their exudation in the rhizosphere. The GC-MS analysis of metabolites revealed that the bacterial inoculation in plants amplified the abundance of flavonoids, terpenes and glucoside metabolites in the roots, which also exuded into the rhizosphere. Flavonoids were the most common prevalent metabolite group in individual and consortium inoculants, highlighting their role as key metabolites in interactions with rhizosphere microbes. In addition, the bacterial inoculants significantly increased antioxidant activity as well as total phenolic and flavonoid content in the leaves of . In conclusion, we propose a model of circular metabolic communication in which rhizosphere bacteria induce the production of flavonoids in plants. In turn, the plant releases some of these flavonoids into the rhizosphere to support the indigenous microbial community for its own benefit.
植物向根际释放大量代谢物，这些代谢物会影响微生物群落的组成和活性。反过来，这些群落又会影响宿主植物的生长和代谢。药用植物与其相关微生物之间的联系已被提及，但本土微生物的选择机制及其在药用植物中的生物学功能在很大程度上仍不清楚。在本研究中，我们调查了植物如何选择其根际细菌，并研究了它们对植物有益的功能作用。我们使用了两种促进植物生长的根际细菌（PGPR）菌株： sp. GLAU - BT2和 sp. GLAU - BT16，将其作为单一或混合接种剂用于植物生长实验。我们分析了在单一和混合细菌接种剂存在下的非靶向根系代谢物，并证实了它们在根际的分泌。代谢物的气相色谱 - 质谱分析表明，接种细菌后， 植物根系中黄酮类、萜类和糖苷类代谢物的丰度增加，这些代谢物也分泌到根际。黄酮类是单一和混合接种剂中最常见的代谢物组，突出了它们作为与根际微生物相互作用的关键代谢物的作用。此外，接种细菌显著提高了 植物叶片的抗氧化活性以及总酚和黄酮含量。总之，我们提出了一个循环代谢通讯模型，其中根际细菌诱导植物中黄酮类化合物的产生。反过来，植物将其中一些黄酮类化合物释放到根际，以支持本土微生物群落，从而使自身受益。
