通过离子交换液相色谱法测定3-脱氧-D-甘露糖辛酸（KDO）、N-乙酰神经氨酸及其衍生物。

Determination of 3-deoxy-D-manno-octulosonic acid (KDO), N-acetylneuraminic acid, and their derivatives by ion-exchange liquid chromatography.

作者信息

Shukla A K, Schauer R, Unger F M, Zähringer U, Rietschel E T, Brade H

出版信息

Carbohydr Res. 1985 Jul 1;140(1):1-8. doi: 10.1016/0008-6215(85)85044-8.

Abstract

A liquid chromatography (1.6 MPa) system for the analysis of 3-deoxy-D-manno-2-octulosonic acid (KDO), N-acetylneuraminic acid (Neu5Ac), methyl alpha- and beta-glycosides of Neu5Ac and KDO, alpha-heptosyl-(1----5)-KDO, various sialyllactoses, alpha-KDO-(2----4)-KDO, alpha-KDO-(2----4)-KDO methyl alpha-glycoside, beta-KDO-(2----4)-KDO methyl beta-glycoside, D-glucuronic acid, D-glucurono-3,6-lactone, and D-galacturonic acid has been developed. Separation was achieved within 10 and 30 min by the use of a small column filled with a strongly basic, anion-exchange resin, Aminex A-29, and 0.75 or 10mM sodium sulfate solutions as mobile phases. This method allowed the determination of KDO and sialic acids in amounts of 100 ng (0.5 nmol) and 200 pg (0.6 pmol), respectively.

摘要

已开发出一种用于分析3-脱氧-D-甘露-2-辛酮糖酸（KDO）、N-乙酰神经氨酸（Neu5Ac）、Neu5Ac和KDO的α-和β-甲基糖苷、α-庚糖基-（1→5）-KDO、各种唾液乳糖、α-KDO-（2→4）-KDO、α-KDO-（2→4）-KDO甲基α-糖苷、β-KDO-（2→4）-KDO甲基β-糖苷、D-葡萄糖醛酸、D-葡萄糖醛酸-3,6-内酯和D-半乳糖醛酸的液相色谱（1.6MPa）系统。使用填充有强碱性阴离子交换树脂Aminex A-29的小柱以及0.75或10mM硫酸钠溶液作为流动相，在10至30分钟内实现了分离。该方法分别能够测定100 ng（0.5 nmol）的KDO和200 pg（0.6 pmol）的唾液酸。