Xie Yong-Qiang, Suo Qiang, Jiang Shan, Li Lin, Ren Chun-Yan, Wu Rong, Shi Xiao-Ning, Zhao Xiao-Qiang, Wang Li-Na, Yin Reng-Jie, Chen Hui, Zhang Hao-Bo
Experimental & Training Teaching Centers, College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, People's Republic of China.
School of Public Health, Gansu University of Chinese Medicine, Lanzhou, Gansu, 730000, People's Republic of China.
Photochem Photobiol Sci. 2025 Jul;24(7):1093-1105. doi: 10.1007/s43630-025-00748-1. Epub 2025 Jun 18.
This study details the design and synthesis of a near-infrared (NIR) dual-channel cyanide detection fluorescent probe based on triphenylamine-hemicyanine. TPA-CY successfully utilizes the NIR fluorescence properties of triphenylamine-hemicyanine conjugates, showcasing good selectivity and sensitivity, facilitating the visual monitoring of cyanide anions in living cells. Interaction between TPA-CY and CN occurs rapidly, within a mere 25 s. The sensitivity of TPA-CY to cyanide ions is good, with detection thresholds reaching 0.60 µM and 0.66 µM for UV and fluorescence spectra, respectively. The detection mechanism of this probe is the nucleophilic addition of CN to the C = N bond, which hindered intramolecular charge transfer. Moreover, the probe's efficacy in tracking cyanide radicals in biological systems is validated through live cell imaging.
本研究详细介绍了一种基于三苯胺-半菁的近红外（NIR）双通道氰化物检测荧光探针的设计与合成。TPA-CY成功利用了三苯胺-半菁共轭物的近红外荧光特性，展现出良好的选择性和灵敏度，便于对活细胞中的氰根阴离子进行可视化监测。TPA-CY与CN之间的相互作用迅速，仅需25秒。TPA-CY对氰离子的灵敏度良好，紫外和荧光光谱的检测限分别达到0.60 μM和0.66 μM。该探针的检测机制是CN对C=N键的亲核加成，这阻碍了分子内电荷转移。此外，通过活细胞成像验证了该探针在生物系统中追踪氰自由基的功效。
