Modeel Sonakshi, Chaurasia Meenu, Siwach Sneha, Dolkar Padma, Negi Rakesh Kumar, Negi Ram Krishan
Fish Molecular Biology Lab, Department of Zoology, University of Delhi, North Campus, Delhi, 110007, India.
Department of Biosciences, Himachal Pradesh University, Summer Hill, Shimla, Himachal Pradesh, 171005, India.
Biochem Genet. 2025 Jun 18. doi: 10.1007/s10528-025-11157-5.
The genus Channa, commonly known as Snakeheads comprises a diverse variety of species that hold great significance in the commercial sectors. Their complexity and genetic variety show how adaptable they are to different environmental settings and shed light on their evolutionary history. To delve into the genetic intricacies of the genus Channa, we analyzed mitochondrial genetic diversity using 1372 COI sequences obtained from the Barcode of Life Data System (BOLD) database. The metadata and phylogenetic analysis revealed the presence of species complex within C. gachua and C. marulius, suggesting the potential existence of intra and inter-clades within one species group. Further, we selected four ecologically and economically important taxonomic groups to study their haplotype diversity and genetic differentiation. These species/taxonomic groups include C. striata, C. punctata, gachua species complex, and marulius species complex. The analysis indicated a substantial level of genetic differentiation and haplotype diversity in species groups indicating high gene flow within populations. Mitochondrial introgression and species complexes account for a significant section of errors in DNA barcodes, which are two of the primary challenges associated with employing DNA barcoding to identify species. Highlighting these challenges and ongoing uncertainties in specific taxonomic groups of genus Channa, the study argues that the efficacy of DNA barcoding and the genetic integrity of wild variation may be weakened when speciation results in the establishment of numerous cryptic taxa in a species complex.
鳢属，通常被称为黑鱼，包含多种在商业领域具有重要意义的物种。它们的复杂性和基因多样性显示了它们对不同环境的适应能力，并揭示了它们的进化历史。为了深入研究鳢属的基因复杂性，我们使用从生命条形码数据系统（BOLD）数据库获得的1372个细胞色素氧化酶亚基I（COI）序列分析了线粒体遗传多样性。元数据和系统发育分析揭示了克氏鳢和线鳢中存在物种复合体，这表明在一个物种组内可能存在支内和支间的情况。此外，我们选择了四个具有生态和经济重要性的分类群来研究它们的单倍型多样性和遗传分化。这些物种/分类群包括条纹鳢、斑鳢、克氏鳢物种复合体和线鳢物种复合体。分析表明，物种组中存在相当程度的遗传分化和单倍型多样性，表明种群内基因流动较高。线粒体渐渗和物种复合体在DNA条形码错误中占很大一部分，这是使用DNA条形码识别物种所面临的两个主要挑战。该研究强调了鳢属特定分类群中这些挑战和持续的不确定性，认为当物种形成导致在一个物种复合体中建立众多隐存分类单元时，DNA条形码的有效性和野生变异的遗传完整性可能会被削弱。
