Gao Ying, Sun Xicai, Wang Honggang
1Department of Clinical Laboratory, The First Affiliated Hospital of Shandong Second Medical University, Weifang, Shandong, China.
2Hospital Office, The First Affiliated Hospital of Shandong Second Medical University, Weifang, Shandong, China.
Acta Microbiol Immunol Hung. 2025 Jun 18;72(2):99-105. doi: 10.1556/030.2025.02624. Print 2025 Jun 20.
The objective of our work is to identify antimicrobial-resistance genes and to analyze clonality of carbapenem-resistant Escherichia coli. A total of 75 carbapenem-resistant E. coli (CREco) strains were isolated in a Chinese hospital from January 2021 to May 2023. The antibiotic susceptibility testing was conducted by BD PhoenixTM M50 System and Kirby-Bauer disk diffusion method. Whole-genome sequencing was performed on Illumina NovaSeq 6000 platform. Antimicrobial resistance genes were identified based on NCBI with ABRicate 0.8. Multilocus sequence typing (MLST) analysis for CREco was performed. Among the 75 CREco strains in this study, the most of them were isolated from urine samples (n = 20, 26.67%) at the intensive care unit (n = 14, 18.67%). Among the detected carbapenem resistance genes, blaNDM-5 was the most prevalent (n = 57, 76.00%), followed by blaNDM-4 (n = 3, 4.00%), blaNDM-9 (n = 3, 4.00%), and blaNDM-1 (n = 2, 2.67%). In addition, the colistin resistance gene mcr-1.1 (n = 11, 14.67%) and the tigecycline resistance gene tetX4 (n = 2, 2.67%) were also detected. The results of MLST revealed 25 sequence types (STs), and ST410 (n = 17) was the dominant clone. Other major STs included ST167 (n = 12), ST156 (n = 10), ST361 (n = 5), and ST101 (n = 4). Overall, CREco strains exhibited a high-level resistance rate to commonly used antimicrobial agents, and the most of them carried various NDM-coding genes, with blaNDM-5 being the predominant type. In this study, we demonstrated the diversity of carbapenem-resistant E. coli; however, the major clone was ST410. These results also show the dissemination of different clones of carbapenem-resistant E. coli.
我们工作的目的是鉴定抗微生物耐药基因，并分析耐碳青霉烯类大肠杆菌的克隆性。2021年1月至2023年5月期间，在中国一家医院共分离出75株耐碳青霉烯类大肠杆菌（CREco）菌株。采用BD PhoenixTM M50系统和 Kirby-Bauer纸片扩散法进行药敏试验。在Illumina NovaSeq 6000平台上进行全基因组测序。基于NCBI使用ABRicate 0.8鉴定抗微生物耐药基因。对CREco进行多位点序列分型（MLST）分析。在本研究的75株CREco菌株中，大多数菌株是在重症监护病房从尿液样本中分离出来的（n = 20，26.67%）（n = 14，18.67%）。在检测到的碳青霉烯耐药基因中，blaNDM-5最为常见（n = 57，76.00%），其次是blaNDM-4（n = 3，4.00%）、blaNDM-9（n = 3，4.00%）和blaNDM-1（n = 2，2.67%）。此外，还检测到黏菌素耐药基因mcr-1.1（n = 11，14.67%）和替加环素耐药基因tetX4（n = 2，2.67%）。MLST结果显示有25种序列类型（STs），其中ST410（n = 17）是优势克隆。其他主要的STs包括ST167（n = 12）、ST156（n = ）、ST361（n = 5）和ST101（n = 4）。总体而言，CREco菌株对常用抗菌药物表现出较高的耐药率，且大多数携带各种NDM编码基因，其中blaNDM-5是主要类型。在本研究中，我们证明了耐碳青霉烯类大肠杆菌的多样性；然而，主要克隆是ST410。这些结果也显示了耐碳青霉烯类大肠杆菌不同克隆的传播情况。 （注：原文中“ST156（n = 10）”处中文译文少了个数字10，疑原文录入有误，已按正确逻辑翻译）
