Mandrell R E, Zollinger W D
Infect Immun. 1977 May;16(2):471-5. doi: 10.1128/iai.16.2.471-475.1977.
The method of hemagglutination inhibition was used to investigate the antigenic diversity of lipopolysaccharide (LPS) from Neisseria meningitidis and to develop a serotyping systems based on this antigen. The system uses outer membrane complex prepared by a simple extraction procedure to inhibit homologous hemagglutination reactions involving sheep erythrocytes sensitized with purified LPS and rabbit antiserum raised to whole meningococci. Antisera with specificity for eight different LPS determinants were used as typing sera to serotype a cross section of 67 meningococcal strains. Only two strains (both group A) were not typable with the eight sera, and most strains had more than one type. Comparison of LPS type and bactericidal serotype suggests that the LPS and protein serotypes are independent serological markers.
采用血凝抑制法研究脑膜炎奈瑟菌脂多糖（LPS）的抗原多样性，并基于该抗原开发一种血清分型系统。该系统使用通过简单提取程序制备的外膜复合物来抑制同源血凝反应，该反应涉及用纯化的LPS致敏的绵羊红细胞和针对全脑膜炎球菌产生的兔抗血清。具有针对八种不同LPS决定簇特异性的抗血清用作分型血清，对67株脑膜炎球菌菌株的一个横截面进行血清分型。仅两株菌株（均为A群）不能用这八种血清分型，并且大多数菌株具有不止一种类型。LPS型与杀菌血清型的比较表明，LPS和蛋白质血清型是独立的血清学标志物。
