Liu Yong, Wang Jie, Zuo Chongkun, Fan Weishu, Yuan Cheng, Zeng Jianmin, Yu Haiqin, Tong Zhijun, Sui Xueyi, Xu Yi, Zhu Min, Tao Xiaorong, Chen Jiongjiong, Kuang Hanhui, Novikova Polina Yu, Huang Changjun
Key Laboratory of Tobacco Biotechnological Breeding, Yunnan Daguan Laboratory, China Tobacco Breeding Technology Innovation Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming 650021, China.
Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Cologne 50829, Germany.
Sci Adv. 2025 Jun 20;11(25):eadw4333. doi: 10.1126/sciadv.adw4333. Epub 2025 Jun 18.
Linkage drag can hinder the integration of resistance genes from wild crop relatives into breeding programs. We used a chromosome-scale genome assembly and a segregating population exceeding 160,000 plants to dissect the complex genetic architecture and overcome the tight linkage between resistance and deleterious loci to produce plants free from linkage drag. We cloned , encoding an immune receptor that confers broad-spectrum resistance to orthotospoviruses through the interaction of its carboxyl-terminal domain with an orthotospovirus-encoded protein. Notably, despite recognizing the same avirulence factor, genes from and from have evolved independently of adjacent nonorthologous ancestral loci. Our work illustrates the potential of wild relative genomes as resources from which to precisely introduce disease resistance into cultivated crops.
连锁累赘会阻碍野生作物近缘种的抗性基因融入育种计划。我们利用染色体水平的基因组组装和一个超过16万株植物的分离群体，剖析复杂的遗传结构，克服抗性位点与有害位点之间的紧密连锁，从而培育出无连锁累赘的植株。我们克隆了 ，其编码一种免疫受体，该受体通过其羧基末端结构域与正番茄斑萎病毒编码的蛋白相互作用，赋予对正番茄斑萎病毒的广谱抗性。值得注意的是，尽管 基因和 基因识别相同的无毒因子，但它们分别来自 和 ，是独立于相邻的非直系同源祖先基因座进化而来的。我们的工作说明了野生近缘种基因组作为资源，从中精确地将抗病性引入栽培作物的潜力。
