Smith M E, Larcom L L, Freeman S E
Chem Biol Interact. 1985 Aug-Sep;54(3):325-35. doi: 10.1016/s0009-2797(85)80173-3.
Freshly isolated human lymphocytes were used to determine how serum supplements affect cellular capacity to repair UV damage. Repair capacity was always found to be greatest in medium supplemented with autologous plasma. Variability in repair capacity among individuals was greater in serum supplemented medium than in unsupplemented medium. Thus, in vitro cellular responses will most accurately represent in vivo responses if autologous serum factors are present in the culture medium. This is of particular importance in studies attempting to correlate DNA repair capacity with age or susceptibility to carcinogenesis.
新鲜分离的人淋巴细胞被用于确定血清补充剂如何影响细胞修复紫外线损伤的能力。在补充了自体血浆的培养基中，修复能力总是被发现是最强的。在补充血清的培养基中，个体间修复能力的变异性比未补充的培养基中更大。因此，如果培养基中存在自体血清因子，体外细胞反应将最准确地代表体内反应。这在试图将DNA修复能力与年龄或致癌易感性相关联的研究中尤为重要。
