James M, Mansbridge J, Kidson C
Int J Radiat Biol Relat Stud Phys Chem Med. 1982 May;41(5):547-56. doi: 10.1080/09553008214550621.
The effects of ultraviolet (U.V.) radiation were studied on a cloned line of human neuroblastoma cells in proliferative and differentiated growth modes, the latter being induced by serum deprivation. The neuroblastoma cells were found to be unusually sensitive in comparison with HeLa cells when survival was measured by colony formation in soft agar, the differentiated mode being the most sensitive. Ultraviolet radiation sensitivity was associated with very low DNA repair capacity as measured by DNA repair synthesis and by removal of M. luteus endonuclease-sensitive sites from irradiated DNA. The greater sensitivity of the differentiated cells appeared to be related to a greater degree of DNA damage at a given U.V. dose, resulting from altered cell geometry in the growth mode. The neuroblastoma cells showed little or no post-irradiation inhibition of DNA replication at low U.V. doses, suggesting that it is the repair process rather than the DNA damage which is responsible for inhibiting replication.
研究了紫外线（U.V.）辐射对处于增殖和分化生长模式的人神经母细胞瘤细胞克隆系的影响，后者是通过血清剥夺诱导产生的。当通过软琼脂中的集落形成来测量存活率时，发现神经母细胞瘤细胞与HeLa细胞相比异常敏感，分化模式最为敏感。通过DNA修复合成以及从受辐照DNA中去除藤黄微球菌核酸内切酶敏感位点来测量，紫外线辐射敏感性与非常低的DNA修复能力相关。分化细胞的更高敏感性似乎与在给定紫外线剂量下更大程度的DNA损伤有关，这是由生长模式中细胞几何形状的改变导致的。在低紫外线剂量下，神经母细胞瘤细胞在辐照后对DNA复制几乎没有或没有抑制作用，这表明负责抑制复制的是修复过程而非DNA损伤。
