Wang Peng, Sui Qihui, Guo Lili, Deng Xianle, Wen Chao, Chen Yueping, Zhou Yanmin
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.
Poult Sci. 2025 Jun 7;104(9):105411. doi: 10.1016/j.psj.2025.105411.
This study aimed to investigate the effects of feeding broilers basal diets with high-temperature (35°C) stored soybean meal (SBM). A total of 192 one-day-old Arbor Acres Plus broilers (male) were divided into three groups with eight replicates for a 42-d experimental period. The three groups were fed a basal diet with fresh SBM, SBM stored at 35°C for 2 and 4 wks, respectively. The results showed that the protein carbonyl content of high-temperature stored SBM increased linearly (P = 0.083) and the protein free thiols content decreased (P = 0.021). Compared with the control group, SBM stored at high temperatures for 4 wks decreased the average daily gain (P = 0.002) and body weight (P = 0.002) of broilers, and increased the feed conversion ratio of 1-21 d (P = 0.008). The high-temperature stored SBM for 4 wks increased the 21-d ileal trypsin activity (P = 0.047), duodenal carbonyl content (P = 0.012), jejunal reactive oxygen species levels (P = 0.047) and 42-d duodenal and jejunal ROS levels of broilers (P = 0.029). The high-temperature stored SBM for 4 wks decreased the 21-d duodenal total superoxide dismutase activity (P = 0.003) and catalase activity (P = 0.006). Moreover, The high-temperature stored SBM altered intestinal chyme composition, increased 42-d jejunal methionine sulfoxide and cystine levels, and decreased L-leucine and lysine levels. These changes have caused 114 DEPs in the jejunum of broilers, there were 53 up-regulated and 61 down-regulated. Furthermore, the upregulated MAPK14 significantly affected the downstream transcription factors c-JUN and FoxO1. Our findings revealed the effects and mechanisms by which intestinal function was affected after broiler chickens ingested protein-oxidized SBM and identified the composition changes in intestinal chyme, DEPs and signaling pathways in the intestinal tissue.
本研究旨在探讨给肉鸡饲喂基础日粮中添加高温（35°C）储存豆粕（SBM）的效果。总共192只1日龄的爱拔益加肉鸡（雄性）被分为三组，每组8个重复，进行为期42天的试验期。三组分别饲喂含有新鲜SBM、在35°C储存2周和4周的SBM的基础日粮。结果表明，高温储存SBM的蛋白质羰基含量呈线性增加（P = 0.083），蛋白质游离巯基含量降低（P = 0.021）。与对照组相比，在高温下储存4周的SBM降低了肉鸡的平均日增重（P = 0.002）和体重（P = 0.002），并提高了1 - 21日龄的饲料转化率（P = 0.008）。高温储存4周的SBM提高了肉鸡21日龄回肠胰蛋白酶活性（P = 0.047）、十二指肠羰基含量（P = 0.012）、空肠活性氧水平（P = 0.047）以及42日龄十二指肠和空肠的ROS水平（P = 0.029）。高温储存4周的SBM降低了21日龄十二指肠总超氧化物歧化酶活性（P = 0.003）和过氧化氢酶活性（P = 0.006）。此外，高温储存的SBM改变了肠道食糜组成，增加了42日龄空肠蛋氨酸亚砜和胱氨酸水平，并降低了L - 亮氨酸和赖氨酸水平。这些变化导致肉鸡空肠中有114个差异表达蛋白（DEP），其中53个上调，61个下调。此外，上调的丝裂原活化蛋白激酶14（MAPK14）显著影响下游转录因子c - JUN和叉头框蛋白O1（FoxO1）。我们的研究结果揭示了肉鸡摄入蛋白质氧化的SBM后肠道功能受到影响的作用和机制，并确定了肠道食糜组成、DEP以及肠道组织中信号通路的变化。
